Biochemistry (Moscow)

, Volume 76, Issue 3, pp 339–346 | Cite as

Foot and mouth disease virus polyepitope protein produced in bacteria and plants induces protective immunity in guinea pigs

  • E. P. Andrianova
  • S. R. Krementsugskaia
  • N. N. Lugovskaia
  • T. K. Mayorova
  • V. V. Borisov
  • M. A. Eldarov
  • N. V. Ravin
  • A. S. FolimonovEmail author
  • K. G. Skryabin


The goal of this project was to develop an alternative foot and mouth disease (FMD) vaccine candidate based on a recombinant protein consisting of efficient viral epitopes. A recombinant gene was designed that encodes B-cell epitopes of proteins VP1 and VP4 and T-cell epitopes of proteins 2C and 3D. The polyepitope protein (H-PE) was produced in E. coli bacteria or in N. benthamiana plants using a phytovirus expression system. The methods of extraction and purification of H-PE proteins from bacteria and plants were developed. Immunization of guinea pigs with the purified H-PE proteins induced an efficient immune response against foot and mouth disease virus (FMDV) serotype O/Taiwan/99 and protection against the disease. The polyepitope protein H-PE can be used as a basis for developing a new recombinant vaccine against FMD.

Key words

FMDV vaccine transient expression in plants polyepitope protein 



cell culture infectious dose 50%


foot and mouth disease


immunoenzyme assay


microneutralization reaction


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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • E. P. Andrianova
    • 1
  • S. R. Krementsugskaia
    • 2
  • N. N. Lugovskaia
    • 2
  • T. K. Mayorova
    • 2
  • V. V. Borisov
    • 2
  • M. A. Eldarov
    • 1
  • N. V. Ravin
    • 1
  • A. S. Folimonov
    • 1
    Email author
  • K. G. Skryabin
    • 1
  1. 1.Bioengineering CenterRussian Academy of SciencesMoscowRussia
  2. 2.Federal State Institution “Federal Center for Animal Health”YuryevetsVladimir, Russia

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