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Biochemistry (Moscow)

, Volume 76, Issue 3, pp 332–338 | Cite as

Amino acid sequences of two immune-dominant epitopes of recoverin are involved in Ca2+/recoverin-dependent inhibition of phosphorylation of rhodopsin

  • I. I. SeninEmail author
  • N. K. Tikhomirova
  • V. A. Churumova
  • I. I. Grigoriev
  • T. A. Kolpakova
  • D. V. Zinchenko
  • P. P. Philippov
  • E. Yu. Zernii
Article

Abstract

Antibodies AB60–72 and AB80–92 against two immune-dominant epitopes of photoreceptor Ca2+-binding protein recoverin, 60-DPKAYAQHVFRSF-72 and 80-LDFKEYVIALHMT-92, which can be exposed in a Ca2+-dependent manner, were obtained. The presence of AB60–72 or AB80–92 results in a slight increase in Ca2+-affinity of recoverin and does not affect significantly a Ca2+-myristoyl switch mechanism of the protein. However in the presence of AB60–72 or AB80–92 recoverin loses its ability to interact with rhodopsin kinase and consequently to perform a function of Ca2+-sensitive inhibitor of rhodopsin phosphorylation in photoreceptor cells.

Key words

Ca2+-dependent antibodies Ca2+-myristoyl switch cancer associated retinopathy recoverin 

Abbreviations

AB60–72 and AB80–92

antibodies against peptide fragments of recoverin 60-DPKAYAQHVFRSF-72 and 80-LDFKEYVIALHMT-92, respectively

CAR

cancer associated retinopathy

DTT

dithiothreitol

PMSF

phenylmethylsulfonyl fluoride

ROS

rod outer segment

TCA

trichloroacetic acid

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • I. I. Senin
    • 1
    Email author
  • N. K. Tikhomirova
    • 1
  • V. A. Churumova
    • 1
  • I. I. Grigoriev
    • 1
  • T. A. Kolpakova
    • 1
  • D. V. Zinchenko
    • 2
  • P. P. Philippov
    • 1
  • E. Yu. Zernii
    • 1
  1. 1.Department of Cell Signaling, Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Laboratory of Protein ChemistryPushchino Branch of the Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryPushchino, Moscow RegionRussia

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