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Biochemistry (Moscow)

, Volume 76, Issue 2, pp 196–201 | Cite as

Interaction between MAK-V protein kinase and synaptopodin

  • S. V. KalinichenkoEmail author
  • P. N. Vikhreva
  • I. V. Korobko
Article

Abstract

MAK-V protein kinase (also known as HUNK) was discovered more than decade ago but its functions and molecular mechanisms of action still remain mostly unknown. In an attempt to associate MAK-V with particular chains of molecular events, we searched for proteins interacting with the C-terminal domain of MAK-V protein kinase. We identified synaptopodin as a protein interaction partner for MAK-V and confirmed this interaction in various ways. Because synaptopodin is important for dendritic spine formation and plays a role in synaptic plasticity, our results might have significant impact on future studies for understanding the role of MAK-V in cells of the nervous system.

Key words

MAK-V/HUNK protein kinase synaptopodin protein-protein interactions brain 

Abbreviations

GAL4AD

transcription activation domain of GAL4 transcription factor

GAL4BD

DNA-binding domain of GAL4 transcription factor

GFP

green fluorescent protein

GST

glutathione S-transferase

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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • S. V. Kalinichenko
    • 1
    Email author
  • P. N. Vikhreva
    • 1
  • I. V. Korobko
    • 1
  1. 1.Institute of Gene BiologyRussian Academy of SciencesMoscowRussia

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