Biochemistry (Moscow)

, Volume 76, Issue 1, pp 118–130 | Cite as

Mechanism of recognition and repair of damaged DNA by human 8-oxoguanine DNA glycosylase hOGG1

  • N. A. Kuznetsov
  • V. V. Koval
  • O. S. FedorovaEmail author


Recent data on structural and biochemical features of human 8-oxoguanine DNA glycosylase (hOGG1) has enabled detailed evaluation of the mechanism by which the damaged DNA bases are recognized and eliminated from the chain. Pre-steady-state kinetic studies with recording of conformational transitions of the enzyme and DNA substrate significantly contribute to understanding of this mechanism. In this review we particularly focus on the interrelationship between the conformational changes of interacting molecules and kinetics of their interaction and on the nature of each elementary step during the enzymatic process. Exhaustive analysis of these data and detailed mechanism of hOGG1-catalyzed reaction are proposed.

Key words

conformational dynamics pre-steady-state kinetics human 8-oxoguanine DNA glycosylase hOGG1 



apurinic/apyrimidinic site




DNA base excision repair

Cy3 and Cy5

cyanine dyes


DABCYL; F, 2-(hydroxymethyl)-3-hydroxytetrahydrofuran




fluorescence resonance energy transfer




human 8-oxoguanine DNA glycosylase




reactive oxygen species




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Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • N. A. Kuznetsov
    • 1
  • V. V. Koval
    • 1
  • O. S. Fedorova
    • 1
    Email author
  1. 1.Institute of Chemical Biology and Fundamental MedicineSiberian Branch of the Russian Academy of SciencesNovosibirskRussia

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