Enzymes of SPZ7 phage: Isolation and properties
- 73 Downloads
Bacteriophage enzyme preparations exolysin and endolysin were studied. Exolysin (a phage-associated enzyme) was obtained from tail fraction and endolysin from phage-free cytoplasmic fraction of disintegrated Salmonella enteritidis cells. A new method for purification of these enzymes was developed, and their molecular masses were determined. The main catalytic properties of the studied enzymes (pH optimum and specificity to bacterial substrates) were found to be similar. Both enzymes lyse Escherichia coli cells like chicken egg lysozyme, but more efficiently lyse S. enteritidis cells and cannot lyse Micrococcus luteus, a good substrate for chicken egg lysozyme. Similar properties of exolysin and endolysin suggest that these enzymes are structurally similar or even identical.
Key wordsbacteriophage enzyme lysis of bacteria bacteriolytic activity
bovine serum albumin
fish-flour pancreatic hydrolyzate
Unable to display preview. Download preview PDF.
- 5.Adams, M. (1961) Bacteriophages [Russian translation], Izd-vo Inostrannoi Literatury, Moscow.Google Scholar
- 9.Velick, S. F., and Furfine, C. (1963) Glyceraldehyde 3-Phosphate Dehydrogenase, in The Enzymes (Boyer, P. D., Lardy, H., and Myrback, K., eds.) Vol. 7, 2nd Edn., Academic Press, NY, pp. 243–273.Google Scholar
- 10.Severin, S. E., and Solov’eva, G. A. (1989) Handbook on Biochemistry [in Russian], Lomonosov Moscow State University, Moscow.Google Scholar
- 11.Osterman, L. A. (1985) Chromatography of Proteins and Nucleic Acids [in Russian], Nauka, Moscow.Google Scholar
- 12.Zuev, V. A. (1969) Lytic Activity of Bacterial Viruses [in Russian], Meditsina, Moscow.Google Scholar