Biochemistry (Moscow)

, Volume 75, Issue 9, pp 1148–1152 | Cite as

Identification of proteins overexpressed in papillary thyroid tumors

  • L. V. Sipina
  • Yu. A. Bukurova
  • I. G. Nikitina
  • G. S. Krasnov
  • S. A. Sergeev
  • N. A. Lisitsyn
  • V. L. Karpov
  • S. F. Beresten
Article

Abstract

A modified method of proteome comparative analysis based on preliminary removal of cell structural proteins by extraction using salt buffer and subsequent separation of extracts by two-dimensional gel electrophoresis was developed. Identification of differentially expressed proteins by mass spectrometry has revealed three proteins with noticeably increased level of synthesis in most samples of papillary thyroid tumors compared to normal tissues. An increase in ubiquitin content was found for the first time. Oncomarker search efficiencies by two-dimensional gel electrophoresis and bioinformatic search were compared.

Key words

differential proteomics two-dimensional gel electrophoresis thyroid cancer protein oncomarkers 

Abbreviations

EST

expressed mRNA sequences

IHCh

immunohistochemistry

MALDI-TOF mass spectrometry

time-of-flight mass spectrometry with laser desorption/deionization

TC

thyroid cancer

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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • L. V. Sipina
    • 1
  • Yu. A. Bukurova
    • 2
  • I. G. Nikitina
    • 2
  • G. S. Krasnov
    • 2
  • S. A. Sergeev
    • 3
  • N. A. Lisitsyn
    • 2
  • V. L. Karpov
    • 2
  • S. F. Beresten
    • 2
  1. 1.Clinical Research Center PreMedMoscowRussia
  2. 2.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia
  3. 3.City Oncology Hospital No. 62Stepanovskoe, Krasnogorsk District, Moscow RegionRussia

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