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Biochemistry (Moscow)

, Volume 75, Issue 9, pp 1139–1147 | Cite as

Influence of ATP-dependent K+-channel opener on K+-cycle and oxygen consumption in rat liver mitochondria

  • O. V. AkopovaEmail author
  • V. I. Nosar
  • V. A. Bouryi
  • I. N. Mankovskaya
  • V. F. Sagach
Article

Abstract

The influence of the K ATP + -channel opener diazoxide on the K+ cycle and oxygen consumption has been studied in rat liver mitochondria. It was found that diazoxide activates the K ATP + -channel in the range of nanomolar concentrations (50–300 nM, K 1/2 ∼ 140 nM), which results in activation of K+/H+ exchange in mitochondria. The latter, in turn, accelerates mitochondrial respiration in respiratory state 2. The contribution of K ATP + -channel to the mitochondrial potassium cycle was estimated using the selective K ATP + -channel blocker glibenclamide. The data show that the relative contribution of K ATP + -channel in the potassium cycle of mitochondria is variable and increases only with the decrease in the ATP-independent component of K+ uptake. Possible mechanisms underlying the observed phenomena are discussed. The experimental results more fully elucidate the role of K ATP + -channel in the regulation of mitochondrial functions, especially under pathological conditions accompanied by impairment of the mitochondrial energy state.

Key words

ATP-dependent K+-channel mitochondria potassium transport diazoxide oxygen consumption potassium cycle 

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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • O. V. Akopova
    • 1
    Email author
  • V. I. Nosar
    • 1
  • V. A. Bouryi
    • 1
  • I. N. Mankovskaya
    • 1
  • V. F. Sagach
    • 1
  1. 1.Bogomolets Institute of PhysiologyNational Academy of Sciences of UkraineKievUkraine

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