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Biochemistry (Moscow)

, Volume 75, Issue 7, pp 881–891 | Cite as

Expression of G-protein coupled receptors in Escherichia coli for structural studies

  • L. E. PetrovskayaEmail author
  • A. A. Shulga
  • O. V. Bocharova
  • Ya. S. Ermolyuk
  • E. A. Kryukova
  • V. V. Chupin
  • M. J. J. Blommers
  • A. S. Arseniev
  • M. P. Kirpichnikov
Article

Abstract

To elaborate a high-performance system for expression of genes of G-protein coupled receptors (GPCR), methods of direct and hybrid expression of 17 GPCR genes in Escherichia coli and selection of strains and bacteria cultivation conditions were investigated. It was established that expression of most of the target GPCR fused with the N-terminal fragment of OmpF or Mistic using media for autoinduction provides high output (up to 50 mg/liter).

Key words

autoinduction expression in E. coli GPCR OmpF Mistic 

Abbreviations

GPCR

G-protein coupled receptors

GST

glutathione-S-transferase

IPTG

isopropyl-β-D-thiogalactoside

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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • L. E. Petrovskaya
    • 1
    Email author
  • A. A. Shulga
    • 1
  • O. V. Bocharova
    • 1
  • Ya. S. Ermolyuk
    • 1
  • E. A. Kryukova
    • 1
  • V. V. Chupin
    • 1
  • M. J. J. Blommers
    • 2
  • A. S. Arseniev
    • 1
  • M. P. Kirpichnikov
    • 1
    • 3
  1. 1.Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Novartis Institutes for BioMedical ResearchBaselSwitzerland
  3. 3.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia

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