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Biochemistry (Moscow)

, Volume 75, Issue 5, pp 629–635 | Cite as

Regulatory protein Yap1 is involved in response of yeast Saccharomyces cerevisiae to nitrosative stress

  • O. V. Lushchak
  • Y. Inoue
  • V. I. LushchakEmail author
Article

Abstract

The goal of this work was to investigate the possible involvement of protein transcription factor Yap1 in regulation of activity of antioxidant enzymes superoxide dismutase and catalase during yeast response to nitrosative stress. It was found that the inactivation of the YAP1 gene, encoding Yap1p, cancelled the activation of superoxide dismutase and catalase by NO-donors. Then, using chimeric protein Yap1-GFP, we found the accumulation of Yap1p in the nucleus in response to nitrosative stress. Therefore, we conclude that these results in combination with previous data clearly demonstrate the involvement of Yap1p in upregulation of superoxide dismutase and catalase in yeast cells in response to nitrosative stress.

Key words

yeast Saccharomyces cerevisiae nitrosative stress Yap1p regulatory protein fluorescence 

Abbreviations

GFP

green fluorescent protein

G6PDH

glucose-6-phosphate dehydrogenase

GR

glutathione reductase

GSH/GSSG

reduced/oxidized glutathione

GSNO

S-nitrosoglutathione

IDH

isocitrate dehydrogenase

(i)NOS

(inducible) NO-synthase

MDH

malate dehydrogenase

ROS (RNS)

reactive oxygen (nitrogen) species

SNP

sodium nitro-prusside

SOD

superoxide dismutase

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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  1. 1.Department of BiochemistryVassyl Stefanyk Precarpathian National UniversityIvano-FrankivskUkraine
  2. 2.Division of Applied Life SciencesGraduate School of AgricultureKyotoJapan

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