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Biochemistry (Moscow)

, Volume 75, Issue 5, pp 606–613 | Cite as

Capsular polysaccharide of the bacterium Azospirillum lipoferum Sp59b: Structure and antigenic specificity

  • O. N. Smol’kinaEmail author
  • V. V. Kachala
  • Yu. P. Fedonenko
  • G. L. Burygin
  • E. L. Zdorovenko
  • L. Yu. Matora
  • S. A. Konnova
  • V. V. Ignatov
Article

Abstract

Antigenic differences were revealed between the cell wall outer membrane lipopolysaccharides and the capsular high molecular weight bioglycans for a typical strain of the nitrogen-fixing rhizobacterium Azospirillum lipoferum Sp59b using antibodies prepared against the homologous lipopolysaccharide and lipopolysaccharide-protein complex. From the capsular lipopolysaccharide-protein and polysaccharide-lipid complexes of A. lipoferum Sp59b, polysaccharides were isolated and their structure was for the first time established in Azospirillum by monosaccharide analysis which included determination of the absolute configurations, methylation, O-deacetylation, and one- and two-dimensional NMR spectroscopy. The polysaccharides of the capsular complexes were shown to have identical structure of the branched tetrasaccharide repeating unit, which differs from the structure of the O-specific polysaccharide within the outer membrane lipopolysaccharide of this strain.

Key words

Azospirillum capsular antigens polysaccharide structure 

Abbreviations

Ab

antibodies

COSY

correlation spectroscopy

CPS

capsular polysaccharides

DPS

O-deacetylated polysaccharide

EIA

enzyme immunoassay

HMBC

heteronuclear multiple bond correlation

HSQC

heteronuclear single quantum coherence

KDO

2-keto-3-deoxyoctonic acid

LPPC

lipopolysaccharide-protein complex

LPS

lipopolysaccharide (O-antigen)

OPS

O-specific polysaccharide

PS

polysaccharide

PSLC

polysaccharide-lipid complex

ROESY

rotation nuclear Overhauser effect spectroscopy

TOCSY

total correlation spectroscopy

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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • O. N. Smol’kina
    • 1
    Email author
  • V. V. Kachala
    • 2
  • Yu. P. Fedonenko
    • 1
  • G. L. Burygin
    • 1
  • E. L. Zdorovenko
    • 2
  • L. Yu. Matora
    • 1
  • S. A. Konnova
    • 1
    • 3
  • V. V. Ignatov
    • 1
  1. 1.Institute of Biochemistry and Physiology of Plants and MicroorganismsRussian Academy of SciencesSaratovRussia
  2. 2.Zelinsky Institute of Organic ChemistryRussian Academy of SciencesMoscowRussia
  3. 3.Saratov State UniversitySaratovRussia

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