Biochemistry (Moscow)

, Volume 75, Issue 5, pp 606–613 | Cite as

Capsular polysaccharide of the bacterium Azospirillum lipoferum Sp59b: Structure and antigenic specificity

  • O. N. Smol’kinaEmail author
  • V. V. Kachala
  • Yu. P. Fedonenko
  • G. L. Burygin
  • E. L. Zdorovenko
  • L. Yu. Matora
  • S. A. Konnova
  • V. V. Ignatov


Antigenic differences were revealed between the cell wall outer membrane lipopolysaccharides and the capsular high molecular weight bioglycans for a typical strain of the nitrogen-fixing rhizobacterium Azospirillum lipoferum Sp59b using antibodies prepared against the homologous lipopolysaccharide and lipopolysaccharide-protein complex. From the capsular lipopolysaccharide-protein and polysaccharide-lipid complexes of A. lipoferum Sp59b, polysaccharides were isolated and their structure was for the first time established in Azospirillum by monosaccharide analysis which included determination of the absolute configurations, methylation, O-deacetylation, and one- and two-dimensional NMR spectroscopy. The polysaccharides of the capsular complexes were shown to have identical structure of the branched tetrasaccharide repeating unit, which differs from the structure of the O-specific polysaccharide within the outer membrane lipopolysaccharide of this strain.

Key words

Azospirillum capsular antigens polysaccharide structure 





correlation spectroscopy


capsular polysaccharides


O-deacetylated polysaccharide


enzyme immunoassay


heteronuclear multiple bond correlation


heteronuclear single quantum coherence


2-keto-3-deoxyoctonic acid


lipopolysaccharide-protein complex


lipopolysaccharide (O-antigen)


O-specific polysaccharide




polysaccharide-lipid complex


rotation nuclear Overhauser effect spectroscopy


total correlation spectroscopy


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  1. 1.
    Michiels, K. W., Crors, C. L., and Vanderleyden, J. (1991) J. Gen. Microbiol., 137, 2241–2246.Google Scholar
  2. 2.
    Skvortsov, I. M., and Ignatov, V. V. (1998) FEMS Microbiol. Lett., 165, 223–229.CrossRefPubMedGoogle Scholar
  3. 3.
    Zdorovenko, G. M. (1988) Mikrobiol. Zh., 50, 98–107.PubMedGoogle Scholar
  4. 4.
    Ovodov, Yu. S. (2006) Biochemistry (Moscow), 71, 937–954.CrossRefGoogle Scholar
  5. 5.
    Konnova, S. A., Makarov, O. E., Skvortsov, I. M., and Ignatov, V. V. (1994) FEMS Microbiol. Lett., 118, 93–99.CrossRefGoogle Scholar
  6. 6.
    Whitfeld, C., and Roberts, I. S. (1999) Mol. Microbiol., 31, 1307–1319.CrossRefGoogle Scholar
  7. 7.
    Matora, L. Yu., and Shchegolev, S. Yu. (2002) Mikrobiologiya, 71, 211–214.Google Scholar
  8. 8.
    Konnova, O. N., Burygin, G. L., Fedonenko, Yu. P., Matora, L. Yu., Pankin, K. E., Konnova, S. A., and Ignatov, V. V. (2006) Mikrobiologiya, 75, 383–388.Google Scholar
  9. 9.
    Del Gallo, M., Negi, M., and Neyra, C. A. (1989) J. Bacteriol., 171, 3504–3510.PubMedGoogle Scholar
  10. 10.
    Yegorenkova, I. V., Konnova, S. A., Sachuk, V. N., and Ignatov, V. V. (2001) Plant Soil, 231, 275–282.CrossRefGoogle Scholar
  11. 11.
    Lasa, I. (2006) Int. Microbiol., 9, 21–28.PubMedGoogle Scholar
  12. 12.
    Fedonenko, Yu. P., Egorenkova, I. V., Konnova, S. A., and Ignatov, V. V. (2001) Mikrobiologiya, 70, 384–390.Google Scholar
  13. 13.
    Hitchcock, P. J., and Brown, T. M. (1983) J. Bacteriol., 154, 269–277.PubMedGoogle Scholar
  14. 14.
    Tsai, C. M., and Frasch, C. E. (1982) Analyt. Biochem., 119, 115–119.CrossRefPubMedGoogle Scholar
  15. 15.
    Fazekas, St. G., Webster, R. G., and Datyner, A. (1963) Biochim. Byophys. Acta, 71, 377.CrossRefGoogle Scholar
  16. 16.
    Konnova, O. N., Boiko, A. S., Burygin, G. L., Fedonenko, Yu. P., Matora, L. Yu., Konnova, S. A., and Ignatov, V. V. (2008) Mikrobiologiya, 77, 383–388.Google Scholar
  17. 17.
    Cabot, E., and Mayer, M. (1968) in Experimental Immunology [Russian translation], Meditsina, Moscow.Google Scholar
  18. 18.
    Ouchterlony, O., and Nilsson, L.-A. (1979) Handbook Experimental Immunology (Weiz, D. M., ed.) Vol. 1, Alden Press, Oxford, pp. 19–33.Google Scholar
  19. 19.
    Mayer, H., Tharanathan, R. N., and Weckesser, J. (1985) Meth. Microbiol., 18, 157–207.CrossRefGoogle Scholar
  20. 20.
    Fedonenko, Yu. P., Konnova, O. N., Zatonsky, G. V., Shashkov, A. S., Konnova, S. A., Zdorovenko, E. L., Ignatov, V. V., and Knirel, Yu. A. (2004) Carbohydr. Res., 339, 1813–1816.CrossRefPubMedGoogle Scholar
  21. 21.
    Jann, B., and Jann, K. (1990) Curr. Top. Microbiol. Immunol., 150, 19–42.PubMedGoogle Scholar
  22. 22.
    Altman, E., Brisson, J. R., and Perry, M. B. (1987) Eur. J. Biochem., 170, 185–192.CrossRefPubMedGoogle Scholar
  23. 23.
    Beynon, L. M., Richards, J. C., and Perry, M. B. (1991) Carbohydr. Res., 220, 185–193.CrossRefPubMedGoogle Scholar
  24. 24.
    Altman, E., Brisson, J. R., Gagne, S. M., and Perry, M. B. (1992) Eur. J. Biochem., 204, 225–230.CrossRefPubMedGoogle Scholar
  25. 25.
    Bhattacharjee, A. K., Jennings, H. J., and Kenney, C. P. (1978) Biochemistry, 17, 645–651.CrossRefPubMedGoogle Scholar
  26. 26.
    Maitra, S. K., Nachum, R., and Pearson, F. C. (1986) Appl. Environ. Microbiol., 52, 510–514.PubMedGoogle Scholar
  27. 27.
    Parker, J. H., Smith, G. A., Fredrickson, H. L., Vestal, J. R., and White, D. C. (1982) Appl. Environ. Microbiol., 44, 1170–1177.PubMedGoogle Scholar
  28. 28.
    Heike, R. U. S., Freudenberg, M., Weckesser, J., and Mayer, H. (1995) Arch. Microbiol., 164, 280–289.CrossRefGoogle Scholar
  29. 29.
    Busse, H. J., Denner, E. B. M., and Lubitz, W. (1996) J. Biotech., 47, 3–38.CrossRefGoogle Scholar
  30. 30.
    Choma, A., and Komaniecka, I. (2008) Carbohydr. Res., 343, 799–804.CrossRefPubMedGoogle Scholar
  31. 31.
    Konnova, S. A., and Ignatov, V. V. (2003) Biology of Plant-Microbe Interaction (Proc. 11th Int. Congr. on Molecular Plant-Microbe Interaction), Vol. 4 (Tikhonovich, I., Lugtenberg, B., and Provorov, N., eds.) St.-Petersburg, pp. 327–331.Google Scholar
  32. 32.
    Vostrikova, O. P., Kim, N. Yu., Likhatskaya, G. N., Guzev, K. V., Vakorina, T. I., Khomenko, V. A., Novikova, O. D., and Soloveva, T. F. (2006) Bioorg. Khim., 32, 371–383.PubMedGoogle Scholar
  33. 33.
    Jansson, P.-E., Kenne, L., and Widmalm, G. (1989) Carbohydr. Res., 188, 169–191.CrossRefPubMedGoogle Scholar
  34. 34.
    Lipkind, G. M., Shashkov, A. S., Knirel, Y. A., Vinogradov, E. V., and Kochetkov, N. K. (1988) Carbohydr. Res., 175, 59–75.CrossRefPubMedGoogle Scholar
  35. 35.
    Fedonenko, Yu. P., Zdorovenko, E. L., Konnova, S. A., Kachala, V. V., and Ignatov, V. V. (2008) Carbohydr. Res., 343, 2841–2844.CrossRefGoogle Scholar
  36. 36.
    Choma, A., Komaniecka, I., and Sowinski, P. (2009) Carbohydr. Res., 344, 936–939.CrossRefPubMedGoogle Scholar
  37. 37.
    Fedonenko, Yu. P., Konnova, O. N., Zatonsky, G. V., Konnova, S. A., Kocharova, N. A., Zdorovenko, E. L., and Ignatov, V. V. (2005) Carbohydr. Res., 340, 1259–1263.CrossRefPubMedGoogle Scholar
  38. 38.
    Newman, M.-N., Dow, J. M., Molinaro, A., and Parrilli, M. (2007) J. Endotoxin Res., 13, 69–84.CrossRefPubMedGoogle Scholar
  39. 39.
    Chen, Y., Bystricky, P., Adeyeye, J., Panigrahi, P., Ali, A., Johnson, J. A., Bush, C. A., Morris, J. G., and Stine, O. C. (2007) BMC Microbiol., 20, 1–15.Google Scholar
  40. 40.
    MacLean, L. L., Perry, M. B., and Vinogradov, E. (2004) Infect. Immun., 72, 5925–5930.CrossRefPubMedGoogle Scholar
  41. 41.
    Masoud, H., Ho, M., Schollaardt, T., and Perry, M. B. (1997) J. Bacteriol., 179, 5663–5669.PubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • O. N. Smol’kina
    • 1
    Email author
  • V. V. Kachala
    • 2
  • Yu. P. Fedonenko
    • 1
  • G. L. Burygin
    • 1
  • E. L. Zdorovenko
    • 2
  • L. Yu. Matora
    • 1
  • S. A. Konnova
    • 1
    • 3
  • V. V. Ignatov
    • 1
  1. 1.Institute of Biochemistry and Physiology of Plants and MicroorganismsRussian Academy of SciencesSaratovRussia
  2. 2.Zelinsky Institute of Organic ChemistryRussian Academy of SciencesMoscowRussia
  3. 3.Saratov State UniversitySaratovRussia

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