Biochemistry (Moscow)

, Volume 75, Issue 5, pp 590–597 | Cite as

Proteomic analysis of salicylate-induced proteins of pea (Pisum sativum L.) leaves

  • I. A. TarchevskyEmail author
  • V. G. Yakovleva
  • A. M. Egorova


The effect of 50 μM salicylic acid on soluble proteins of pea (Pisum sativum L.) leaves was studied by proteomic analysis. Thirty-two salicylate-induced proteins were found, and 13 of these were identified using MALDI TOF MS. Salicylate-induced increased content was shown for the first time for the family 18 glycoside hydrolase, α-amylase, 33 kDa protein of photosystem II, lipid-desaturase-like protein, and glutamine amidotransferase. Increased content of protective proteins of direct antipathogenic action such as chitinase and β-1,3-glucanases was also noted.

Key words

salicylic acid proteomic investigations salicylate-induced proteins 


Bio-Lyte (pH 3-10)



3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (nonionic detergent)

2-D electrophoresis

two-dimensional electrophoresis


isoelectric focusing


protein that does not cause expression of the pathogen-induced protein PR1 (non-expressor of PR1)


salicylic acid


trichloroacetic acid


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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • I. A. Tarchevsky
    • 1
    Email author
  • V. G. Yakovleva
    • 1
  • A. M. Egorova
    • 1
  1. 1.Kazan Institute of Biochemistry and Biophysics, Kazan Research CenterRussian Academy of SciencesKazanRussia

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