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Biochemistry (Moscow)

, Volume 75, Issue 5, pp 579–584 | Cite as

Electron transfer between exogenous electron donors and reaction center of photosystem 2

  • M. D. MamedovEmail author
  • V. N. Kurashov
  • I. O. Petrova
  • A. A. Zaspa
  • A. Yu. SemenovEmail author
Article

Abstract

Transfer of electrons between artificial electron donors diphenylcarbazide (DPC) and hydroxylamine (NH2OH) and reaction center of manganese-depleted photosystem 2 (PS2) complexes was studied using the direct electrometrical method. For the first time it was shown that reduction of redox-active amino acid tyrosine Y z · by DPC is coupled with generation of transmembrane electric potential difference (δΨ). The amplitude of this phase comprised ∼17% of that of the δΨ phase due to electron transfer between YZ and the primary quinone acceptor QA. This phase is associated with vectorial intraprotein electron transfer between the DPC binding site on the protein-water interface and the tyrosine Y z · . The slowing of ΔΨ decay in the presence of NH2OH indicates effective electron transfer between the artificial electron donor and reaction center of PS2. It is suggested that NH2OH is able to diffuse through channels with diameter of 2.0–3.0 Å visible in PS2 structure and leading from the protein-water interface to the Mn4Ca cluster binding site with the concomitant electron donation to Y z · . Because the dielectrically-weighted distance between the NH2OH binding site and Y z · is not determined, the transfer of electrons from NH2OH to Y z · could be either electrically silent or contribute negligibly to the observed electrogenicity in comparison with hydrophobic donors.

Key words

reaction center photosystem 2 proteoliposomes photopotential diphenylcarbazide hydroxylamine channels 

Abbreviations

(B)RC

(bacterial) reaction center

DCPIP

2,6-dichlorophenolindophenol

DPC

diphenylcarbazide

NH2OH

hydroxylamine

OEC

oxygen-evolving complex

P680

primary electron donor

PS2(-Mn)

PS2 preparations depleted of Mn

QA (QB)

primary (secondary) plastoquinone electron acceptor

TMPD

N,N,N′N′-tetramethyl-p-phenylenediamine

τ

time constant

YZ

redox-active tyrosine of D1 polypeptide

ΔΨ

electric potential difference

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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  1. 1.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Faculty of Bioengineering and BioinformaticsLomonosov Moscow State UniversityMoscowRussia

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