Biochemistry (Moscow)

, Volume 75, Issue 5, pp 549–553 | Cite as

Synthetic water-soluble phenolic antioxidant regulates L-arginine metabolism in macrophages: A possible role of Nrf2/ARE

  • V. O. TkachevEmail author
  • E. B. Menshchikova
  • N. K. Zenkov
  • N. V. Kandalintseva
  • N. N. Volsky


Synthetic water-soluble phenolic antioxidant TS-13 exhibits pronounced anti-inflammatory properties in vivo and induces intracellular signal system Nrf2/ARE. At concentrations 150–1000 μM it inhibits nitric oxide (NO) production in mouse peritoneal macrophages. However, this compound at low concentrations (1–100 μM) paradoxically increases NO production and decreases activity of arginase. These results are indicative of an ambiguous role of NO and its metabolites in the mechanism of development of inflammatory reaction.

Key words

nitric oxide arginase macrophages inflammation antioxidant responsive element Nrf2 TS-13 



antioxidant responsive element




concentration required for 50% inhibition


inducible nitric oxide synthase




nitric oxide


phorbol 12-myristate-13-acetate


sodium 3-(3′-tert-butyl-4′-hydroxyphenyl)propyl thiosulfonate


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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • V. O. Tkachev
    • 1
    • 2
    Email author
  • E. B. Menshchikova
    • 1
  • N. K. Zenkov
    • 1
  • N. V. Kandalintseva
    • 3
  • N. N. Volsky
    • 2
  1. 1.Research Center of Clinical and Experimental MedicineSiberian Branch of the Russian Academy of Medical SciencesNovosibirskRussia
  2. 2.Research Institute of Clinical ImmunologySiberian Branch of the Russian Academy of Medical SciencesNovosibirskRussia
  3. 3.Novosibirsk State Pedagogical UniversityNovosibirskRussia

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