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Biochemistry (Moscow)

, Volume 75, Issue 5, pp 549–553 | Cite as

Synthetic water-soluble phenolic antioxidant regulates L-arginine metabolism in macrophages: A possible role of Nrf2/ARE

  • V. O. TkachevEmail author
  • E. B. Menshchikova
  • N. K. Zenkov
  • N. V. Kandalintseva
  • N. N. Volsky
Article

Abstract

Synthetic water-soluble phenolic antioxidant TS-13 exhibits pronounced anti-inflammatory properties in vivo and induces intracellular signal system Nrf2/ARE. At concentrations 150–1000 μM it inhibits nitric oxide (NO) production in mouse peritoneal macrophages. However, this compound at low concentrations (1–100 μM) paradoxically increases NO production and decreases activity of arginase. These results are indicative of an ambiguous role of NO and its metabolites in the mechanism of development of inflammatory reaction.

Key words

nitric oxide arginase macrophages inflammation antioxidant responsive element Nrf2 TS-13 

Abbreviations

ARE

antioxidant responsive element

DCF

2,7-dichlorofluorescein

IC50

concentration required for 50% inhibition

iNOS

inducible nitric oxide synthase

LPS

lipopolysaccharide

NO

nitric oxide

PMA

phorbol 12-myristate-13-acetate

TS-13

sodium 3-(3′-tert-butyl-4′-hydroxyphenyl)propyl thiosulfonate

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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • V. O. Tkachev
    • 1
    • 2
    Email author
  • E. B. Menshchikova
    • 1
  • N. K. Zenkov
    • 1
  • N. V. Kandalintseva
    • 3
  • N. N. Volsky
    • 2
  1. 1.Research Center of Clinical and Experimental MedicineSiberian Branch of the Russian Academy of Medical SciencesNovosibirskRussia
  2. 2.Research Institute of Clinical ImmunologySiberian Branch of the Russian Academy of Medical SciencesNovosibirskRussia
  3. 3.Novosibirsk State Pedagogical UniversityNovosibirskRussia

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