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Biochemistry (Moscow)

, Volume 75, Issue 4, pp 486–493 | Cite as

Effect of trifluoroethanol on native and acid-induced states of glucose oxidase from Aspergillus niger

  • B. Ahmad
  • S. K. Haq
  • A. Varshney
  • A. A. Moosavi-Movahedi
  • R. H. KhanEmail author
Article

Abstract

We have studied the effect of trifluoroethanol (TFE) on the native (pH 7.0), acid unfolded (pH 2.6), and molten globule (pH 1.4) states of glucose oxidase (GOX) by circular dichroism and fluorescence spectroscopy. In the presence of 50% TFE, at pH 7.0 and 2.6, GOX exhibited a transition from native coiled-coil and acid unfolded state to non-associating α-helical state. Interestingly, at pH 1.4, 15% TFE induced the formation of β-structured intermediate by loss of 1-anilino-8-naphthalenesulfonate binding site and almost all tertiary contacts. The β-structured intermediate converted into open helical conformation on further addition of TFE.

Key words

glucose oxidase molten globule coiled-coil state acid unfolded state trifluoroethanol 

Abbreviations

ANS

1-anilino-8-naphthalenesulfonate

CD

circular dichroism

GdnHCl

guanidine hydrochloride

GOX

glucose oxidase

MG

molten globule

MRE

mean residue ellipticity

TFE

2,2,2-trifluoroethanol

UA

acid-induced unfolded state

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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • B. Ahmad
    • 1
  • S. K. Haq
    • 1
  • A. Varshney
    • 1
  • A. A. Moosavi-Movahedi
    • 2
  • R. H. Khan
    • 1
    Email author
  1. 1.Interdisciplinary Biotechnology UnitAligarh Muslim UniversityAligarhIndia
  2. 2.Institute of Biochemistry and BiophysicsUniversity of TehranTehranIran

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