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Biochemistry (Moscow)

, Volume 75, Issue 4, pp 443–451 | Cite as

Biological properties and structure of the lipopolysaccharide of a vaccine strain of Francisella tularensis generated by inactivation of a quorum sensing system gene qseC

  • A. N. Mokrievich
  • A. N. KondakovaEmail author
  • E. Valade
  • M. E. Platonov
  • G. M. Vakhrameeva
  • R. Z. Shaikhutdinova
  • R. I. Mironova
  • D. Blaha
  • I. V. Bakhteeva
  • G. M. Titareva
  • T. B. Kravchenko
  • T. I. Kombarova
  • D. Vidal
  • V. M. Pavlov
  • B. Lindner
  • I. A. Dyatlov
  • Yu. A. Knirel
Article

Abstract

A knockout mutant with a deletion in a quorum sensing system gene qseC was generated from the vaccine strain Francisella tularensis 15 by site-directed mutagenesis. The variant with the inactivated gene qseC differed from the parental strain in growth rate on solid nutrient medium but had the same growth dynamics in liquid nutrient medium. The mutation abolished almost completely the resistance of the vaccine strain to normal rabbit serum and its ability to survive in macrophages; in addition, the strain lost the residual virulence. A significant phenotypic alteration was observed in the lipopolysaccharide of F. tularensis. Particularly, the mutant strain synthesized no noticeable amount of the lipopolysaccharide with the high-molecular-mass O-polysaccharide, presumably as a result of impairing biosynthesis of the repeating unit, namely, a loss of the ability to incorporate a formyl group, an N-acyl substituent of 4-amino-4,6-dideoxy-D-glucose.

Key words

Francisella tularensis tularemia quorum sensing gene qseC site-directed mutagenesis lipopolysaccharide 

Abbreviations

CFU

colony-forming unit

dHexN

deoxyhexosamine

ESI FT ICR

electrospray ionization Fourier-transform ion-cyclotron resonance

GalNAcAN

2-acetamido-2-deoxygalacturonamide

Hex

hexose

HexN

hexosamine

HexNAN

hexosaminuronamide

Kdo

3-deoxy-D-manno-oct-2-ulosonic acid

LPS

lipopolysaccharide

Mr

molecular weight

QS

quorum sensing

QuiNAc

2-acetamido-2,6-dideoxyglu-cose

Qui4N

4-amino-4,6-dideoxyglucose

Qui4NFo

4-form-amido-4,6-dideoxyglucose

3HO18:0

3-hydroxyoctadecanoic acid

16:0

hexadecanoic (palmitic) acid

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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • A. N. Mokrievich
    • 1
  • A. N. Kondakova
    • 2
    • 3
    Email author
  • E. Valade
    • 4
  • M. E. Platonov
    • 1
  • G. M. Vakhrameeva
    • 1
  • R. Z. Shaikhutdinova
    • 1
  • R. I. Mironova
    • 1
  • D. Blaha
    • 4
  • I. V. Bakhteeva
    • 1
  • G. M. Titareva
    • 1
  • T. B. Kravchenko
    • 1
  • T. I. Kombarova
    • 1
  • D. Vidal
    • 4
  • V. M. Pavlov
    • 1
  • B. Lindner
    • 3
  • I. A. Dyatlov
    • 1
  • Yu. A. Knirel
    • 2
  1. 1.State Research Center for Applied Microbiology and BiotechnologyObolensk, Moscow RegionRussia
  2. 2.Zelinsky Institute of Organic ChemistryRussian Academy of SciencesMoscowRussia
  3. 3.Research Center BorstelCenter for Medicine and BiosciencesBorstel, Parkallee 22Germany
  4. 4.Centre de Recherches du Service de Sante des ArmeesLa Tronche CedexFrance

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