Biochemistry (Moscow)

, Volume 75, Issue 4, pp 423–427 | Cite as

Study of effect of molecular mobility in chromatophore membranes of the bacterium E. shaposhnikovii on processes of photoinduced electron transport using the NMR-Spin-Echo method with isotope substitution and dehydration

  • C. S. Chamorovsky
  • S. K. Chamorovsky
  • P. P. KnoxEmail author


The effect of dehydration and 2H2O/H2O isotope substitution on electron transport reactions and relaxation of proton-containing groups was studied in chromatophore membranes of Ectothiorhodospira shaposhnikovii. During dehydration (including isotope substitution of hydrate water) of preliminarily dehydrated isolated photosynthetic membranes there was a partial correlation between hydration intervals within which activation of electron transport from high-potential cytochrome c to photoactive bacteriochlorophyll dimer P890 of photosynthetic reaction center and variation of spin-lattice and spin-spin proton relaxation time was observed. Partial correlation between hydration intervals can be considered as evidence of correlation between mobility of non-water proton-containing groups with proton relaxation frequency ∼108 sec−1 with efficiency of electron transfer at the donor side of the chain.

Key words

electron transport molecular mobility NMR photosynthetic bacteria Ectothiorhodospira shaposhnikovii 



nuclear magnetic resonance


reaction center


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© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • C. S. Chamorovsky
    • 1
  • S. K. Chamorovsky
    • 1
  • P. P. Knox
    • 1
    Email author
  1. 1.Department of Biophysics, Biology FacultyLomonosov Moscow State UniversityMoscowRussia

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