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Biochemistry (Moscow)

, Volume 75, Issue 3, pp 327–335 | Cite as

Probes of inhibition of Escherichia coli F1-ATPase by 7-chloro-4-nitrobenz-2-oxa-1,3-diazole in the presence of MgADP and MgATP support a bi-site mechanism of ATP hydrolysis by the enzyme

  • V. V. Bulygin
  • Y. M. MilgromEmail author
Article

Abstract

Binding of MgADP and MgATP to Escherichia coli F1-ATPase (EcF1) has been assessed by their effects on extent of the enzyme inhibition by 7-chloro-4-nitrobenz-2-oxa-1,3-diazole (NBD-Cl). MgADP at low concentrations (K d 1.3 μM) promotes the inhibition, whereas at higher concentrations (K d 0.7 mM) EcF1 is protected from inhibition. The mutant βY331W-EcF1 requires much higher MgADP, K d of about 10 mM, for protection. Such MgADP binding was not revealed by fluorescence quenching measurements. MgATP partially protects EcF1 from inactivation by NBD-Cl, but the enzyme remains sensitive to NBD-Cl in the presence of MgATP at concentrations as high as 10 mM. The activating anion selenite in the absence of MgATP partially protects EcF1 from inhibition by NBD-Cl. A complete protection of EcF1 from inhibition by NBD-Cl has been observed in the presence of both MgATP and selenite. The results support a bi-site catalytic mechanism for MgATP hydrolysis by F1-ATPases and suggest that stimulation of the enzyme activity by activating anions is due to the anion binding to a catalytic site that remains unoccupied at saturating substrate concentration.

Key words

anion activation ATP synthase catalytic cooperativity bi-site catalysis nucleotide binding tri-site catalysis 

Abbreviations

F1

solubilized portion of FoF1-ATP synthase

MF1, EcF1, and TF1

F1-ATPases from beef-heart mitochondria, Escherichia coli, and thermophilic Bacillus PS3, respectively

NBD-Cl

7-chloro-4-nitrobenz-2-oxa-1,3-diazole

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© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular Biology, State University of New YorkUpstate Medical UniversitySyracuseUSA

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