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Biochemistry (Moscow)

, Volume 75, Issue 3, pp 304–309 | Cite as

Protein kinase C-δ is involved in induction of NOX1 gene expression by aldosterone in rat vascular smooth muscle cells

  • Haiyan Wei
  • Xuhua Mi
  • Ling Ji
  • Lichuan Yang
  • Qingjie Xia
  • Yuquan Wei
  • Isamu Miyamori
  • ChunYuan FanEmail author
Article

Abstract

In this study, we focused on the relationship between aldosterone and NOX1 expression in vascular smooth muscle cells (VSMCs). For the first time, with the use of specific inhibitors of protein kinase C (PKC), we report that PKCδ mediates upregulation of NOX1 induced by 10 nM aldosterone in cultured VSMCs. Participation of PKC in the mediation of NOX1 regulation was further confirmed by the effect of diacylglycerol, a PKC agonist, on the NOX1 RNA in A7r5 cells with Northern blot analysis. To establish cause and effect, we next silenced the PKCδ gene partly by RNA interference and found knockdown of PKCδ gene attenuated aldosterone-induced NOX1 expression, generation of superoxide, as well as protein synthesis in VSMCs. Taken together, these data indicated PKCδ might mediate aldosterone-dependent NOX1 upregulation in VSMCs. In addition, we showed that the cascade from aldosterone to PKCδ activation had the participation of the mineralocorticoid receptor.

Key words

aldosterone NOX1 PKCδ 

Abbreviations

DAG

diacylglycerol

DHE

dihydroethidium

DMEM

Dulbecco’s modified Eagle’s medium

FBS

fetal bovine serum

GAPDH

glyceraldehyde-3-phosphate dehydro-genase

MAPK

mitogen-activated protein kinase

MR

miner-alocorticoid receptor

PKC

protein kinase C

ROS

reactive oxygen species

SSC

standard saline citrate

VSMCs

vascular smooth muscle cells

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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • Haiyan Wei
    • 1
  • Xuhua Mi
    • 2
  • Ling Ji
    • 2
  • Lichuan Yang
    • 2
  • Qingjie Xia
    • 2
  • Yuquan Wei
    • 3
  • Isamu Miyamori
    • 4
  • ChunYuan Fan
    • 2
    Email author
  1. 1.State Key Laboratory of Biotherapy, West China Hospital and School of Life ScienceSichuan UniversityChengduChina
  2. 2.Department of Nephrology, State Key Laboratory of Biotherapy, West China Hospital, West China Medical SchoolSichuan UniversityChengduChina
  3. 3.State Key Laboratory of BiotherapyWest China HospitalChengduChina
  4. 4.Third Department of Internal Medicine, Faculty of Medical SciencesUniversity of FukuiFukuiJapan

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