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Biochemistry (Moscow)

, Volume 75, Issue 2, pp 224–232 | Cite as

Characteristics of binding of zwitterionic liposomes to water-soluble proteins

  • A. S. Dudkina
  • A. A. Selischeva
  • N. I. LarionovaEmail author
Article

Abstract

The interactions of zwitterionic phospholipids phosphatidylcholine and phosphatidylethanolamine with protein proteinase inhibitors aprotinin and Bowman-Birk soybean proteinase inhibitor have been investigated. An increase in the hydrophobicity of the liposome surface was shown to be an important factor for the formation of proteoliposomes. According to 31P-NMR spectra, incorporation of the proteins into the liposomes does not influence the structural organization of the surface of the liposomes. Increasing the ionic strength does not inhibit the process of proteoliposome formation. Fluorescence assay of the complexes of anthracene-labeled phospholipids with the rhodamine B-labeled protein showed that after the encapsulation into the liposomes, the protein is located inside the particles and between the bilayers. Also, the effect of phospholipids with saturated fatty acid residues on the protein-lipid interaction was studied by differential scanning calorimetry. The results indicate that water-soluble proteins efficiently interact with zwitterionic phospholipids, and the encapsulation of the proteins into the liposomes is provided by electrostatic and hydrophobic forces (in the case of aprotinin) or predominantly by hydrophobic forces (Bowman-Birk soybean proteinase inhibitor).

Key words

phosphatidylcholine phosphatidylethanolamine liposomes aprotinin Bowman-Birk soybean proteinase inhibitor differential scanning calorimetry 

Abbreviations

BBI

Bowman-Birk soybean proteinase inhibitor

BPTI

aprotinin

LPC

lysophosphatidylcholine

PC

phosphatidylcholine

PE

phosphatidylethanolamine

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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • A. S. Dudkina
    • 1
  • A. A. Selischeva
    • 2
  • N. I. Larionova
    • 1
    Email author
  1. 1.Chemical FacultyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Biological FacultyLomonosov Moscow State UniversityMoscowRussia

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