Biochemistry (Moscow)

, Volume 75, Issue 1, pp 85–94 | Cite as

Molecular cloning of a cotton phosphatase gene and its functional characterization

  • Shi-You Lu
  • Guang-Rong Zhao
  • Ai-Min Wu
  • M. A. Jenks
  • Shuping Zhang
  • Jin-Yuan Liu
Article

Abstract

In this study, we report isolation of a phosphatase gene designated GhHL1 from cotton and its functional characterization. GhHL1 transcripts were detected in all cotton tissues examined. Southern blotting analysis indicated that it exists in multiple-copies. Biochemical analysis showed that GhHL1 was Mg2+-dependent and cation-sensitive. Purified recombinant GhHL1 protein dephosphorylated both 3′,5′-bisphosphate nucleotide and inositol 1,4-bisphosphate, demonstrating dual 3′,5′-bisphosphate nucleotidase and inositol polyphosphate 1-phosphatase activities. Overexpression of GhHL1 complemented yeast hal2 mutant and enhanced yeast growth under elevated NaCl or LiCl, showing a role in salt tolerance associated with ionic stress response. Taken together, these results show that GhHL1 is a functional and good candidate gene, which might be used to improve salt tolerance in plants.

Key words

cotton phosphatase salt tolerance enzyme activity functional characterization 

Abbreviations

FDD-PCR

fluorescence differential display-polymerase chain reaction

IP2

inositol 1,4-bisphosphate

IP3

inositol 1,4,5-trisphosphate

IPP1ase

inositol polyphosphate 1-phosphatase

PAP

3′(2′),5′-bisphosphate nucleotide

PAPase

3′(2′),5′-bisphosphate nucleotidase

PAPS

3′(2′),5′-bisphosphate nucleotide sulfate

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Copyright information

© Pleiades Publishing, Ltd. 2010

Authors and Affiliations

  • Shi-You Lu
    • 1
  • Guang-Rong Zhao
    • 1
  • Ai-Min Wu
    • 1
  • M. A. Jenks
    • 2
  • Shuping Zhang
    • 1
  • Jin-Yuan Liu
    • 1
  1. 1.Department of Biological Sciences and BiotechnologyTsinghua UniversityBeijingChina
  2. 2.Department of Horticulture and Landscape Architecture, Center for Plant Environmental Stress PhysiologyPurdue UniversityWest LafayetteUSA

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