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Biochemistry (Moscow)

, 74:1285 | Cite as

Isotachophoresis of nucleic acids in agarose gel rods

  • V. N. Kondratova
  • I. V. Botezatu
  • V. P. Shelepov
  • A. V. LichtensteinEmail author
Short Communications

Abstract

A new method of electrophoresis (isotachophoresis in agarose gel rods) in which nucleic acid molecules are not separated but, oppositely, are brought together into one band, was elaborated. Heterogeneous in size DNA and RNA polymers present in a few milliliters of a solution at so low concentration that their isolation by other methods is hardly attainable and fraught with losses are brought together into one visible narrow band when put in a discontinuous electric field. Polynucleotides migrate in dilute (0.1%) semifluid agarose gel that permits easy quantitative isolation of the band of interest. Resulting DNA can be used directly in PCR. The suggested method for isolation of micro amounts of nucleic acids from dilute solutions can be applied to forensic and clinical research and cancer gene diagnostics by the analysis of fragmented circulating DNA from bodily fluids.

Key words

DNA isotachophoresis circulating DNA agarose gel DNA isolation PCR 

Abbreviations

BPB

bromophenol blue

ITP

isotachophoresis

Supplementary material

10541_2009_9117_MOESM1_ESM.pdf (170 kb)
Supplementary material, approximately 170 KB.

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • V. N. Kondratova
    • 1
  • I. V. Botezatu
    • 1
  • V. P. Shelepov
    • 1
  • A. V. Lichtenstein
    • 1
    Email author
  1. 1.Blokhin Russian Cancer Research CenterRussian Academy of Medical SciencesMoscowRussia

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