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Biochemistry (Moscow)

, 74:1270 | Cite as

Identification of messenger RNA substrates for mouse T-STAR

  • L. Y. Zhang
  • M. Zeng
  • P. Chen
  • H. Q. Sun
  • D. C. Tao
  • Y. Q. Liu
  • L. Lin
  • Y. Yang
  • S. Z. Zhang
  • Y. X. MaEmail author
Article

Abstract

Using the method of isolation of specific nucleic acids associated with proteins (SNAAP), we have identified 10 candidate target mRNA substrates bound by mT-STAR (mouse T-STAR protein) from testis extract. Among them, our study focused on Fabp9, a gene that is essential for male gametogenesis, and showed that mT-STAR could directly bind to Fabp9 mRNAs. The binding sites are in a short sequence of the coding region and 3′ untranslated region of Fabp9 mRNA. These suggest that mT-STAR can regulate the metabolism and expression of Fabp9. In conclusion, identification of mT-STAR-bound mRNA substrates might help to illustrate the potential spectrum of the process and provide valuable insight into the biological function of this RNA-binding protein in spermatogenesis.

Key words

Fabp9 mRNA substrates mT-STAR SNAAP spermatogenesis 

Abbreviations

DIG

digoxigenin

hnRNP

heterogeneous nuclear ribonucleoprotein

mT-STAR

mouse T-STAR (testis signal transduction and activation of RNA) protein

RBB

RNA binding buffer

SNAAP

specific nucleic acids associated with proteins

References

  1. 1.
    Hecht, N. B. (1998) Bioessays, 20, 555–561.CrossRefPubMedGoogle Scholar
  2. 2.
    Venables, J. P., and Eperon, I. (1999) Curr. Opin. Genet. Dev., 9, 346–354.CrossRefPubMedGoogle Scholar
  3. 3.
    Lee, J., Hong, J., Kim, E., Kim, K., Kim, S. W., Krishnamurthy, H., Chung, S. S., Wolgemuth, D. J., and Rhee, K. (2004) Mol. Hum. Reprod., 10, 259–264.CrossRefPubMedGoogle Scholar
  4. 4.
    Elliott, D. J., Millar, M. R., Oghene, K., Ross, A., Kiesewetter, F., Pryor, J., McIntyre, M., Hargreave, T. B., Saunders, P. T., Vogt, P. H., Chandley, A. C., and Cooke, H. (1997) Proc. Natl. Acad. Sci. USA, 94, 3848–3853.CrossRefPubMedGoogle Scholar
  5. 5.
    Weighardt, F., Biamonti, G., and Riva, S. (1996) Bioessays, 18, 747–756.CrossRefPubMedGoogle Scholar
  6. 6.
    Kamma, H., Portman, D. S., and Dreyfuss, G. (1995) Exp. Cell Res., 221, 187–196.CrossRefPubMedGoogle Scholar
  7. 7.
    Elliott, D. J. (2004) Int. J. Androl., 27, 328–334.CrossRefPubMedGoogle Scholar
  8. 8.
    Elliott, D. J., Venables, J. P., Newton, C. S., Lawson, D., Boyle, S., Eperon, I. C., and Cooke, H. J. (2000) Hum. Mol. Genet., 9, 2117–2124.CrossRefPubMedGoogle Scholar
  9. 9.
    Stoss, O., Olbrich, M., Hartmann, A. M., Konig, H., Memmott, J., Andreadis, A., and Stamm, S. (2001) J. Biol. Chem., 276, 8665–8673.CrossRefPubMedGoogle Scholar
  10. 10.
    Vernet, C., and Artzt, K. (1997) Trends Genet., 13, 479–484.CrossRefPubMedGoogle Scholar
  11. 11.
    Venables, J. P., Vernet, C., Chew, S. L., Elliott, D. J., Cowmeadow, R. B., Wu, J., Cooke, H. J., Artzt, K., and Eperon, I. C. (1999) Hum. Mol. Genet., 8, 959–969.CrossRefPubMedGoogle Scholar
  12. 12.
    Elliott, D. J., Bourgeois, C. F., Klink, A., Stevenin, J., and Cooke, H. J. (2000) Proc. Natl. Acad. Sci. USA, 97, 5717–5722.CrossRefPubMedGoogle Scholar
  13. 13.
    Venables, J. P., Elliott, D. J., Makarova, O. V., Makarov, E. M., Cooke, H. J., and Eperon, I. C. (2000) Hum. Mol. Genet., 9, 685–694.CrossRefPubMedGoogle Scholar
  14. 14.
    Paronetto, M. P., Achsel, T., Massiello, A., Chalfant, C. E., and Sette, C. (2007) J. Cell Biol., 176, 929–939.CrossRefPubMedGoogle Scholar
  15. 15.
    Trifillis, P., Day, N., and Kiledjian, M. (1999) RNA, 5, 1071–1082.CrossRefPubMedGoogle Scholar
  16. 16.
    King, P. H. (2000) Nucleic Acids Res., 28, E20.CrossRefPubMedGoogle Scholar
  17. 17.
    Pouresmaeili, F., Morales, C. R., and Oko, R. (1997) Biol. Reprod., 57, 655–659.CrossRefPubMedGoogle Scholar
  18. 18.
    Kogami, T., Miki, Y., Yamada, T., Umegaki, T., Nishimura, M., Amo, T., Kosaka, J., and Sasaki, J. (2006) Acta Histochem. Cytochem., 39, 183–192.CrossRefPubMedGoogle Scholar
  19. 19.
    Kool, J., van Zaane, W., van der Eb, A., and Terleth, C. (2001) Cell Growth Differ., 12, 535–541.PubMedGoogle Scholar
  20. 20.
    Hao, Z., Jha, K. N., Kim, Y. H., Vemuganti, S., Westbrook, V. A., Chertihin, O., Markgraf, K., Flickinger, C. J., Coppola, M., Herr, J. C., and Visconti, P. E. (2004) Mol. Hum. Reprod., 10, 433–444.CrossRefPubMedGoogle Scholar
  21. 21.
    Kueng, P., Nikolova, Z., Djonov, V., Hemphill, A., Rohrbach, V., Boehlen, D., Zuercher, G., Andres, A. C., and Ziemiecki, A. (1997) J. Cell Biol., 139, 1851–1859.CrossRefPubMedGoogle Scholar
  22. 22.
    Maeda, T., Nishida, J., and Nakanishi, Y. (1999) Dev. Growth Differ., 41, 715–722.CrossRefPubMedGoogle Scholar
  23. 23.
    Gibbs, G. M., Scanlon, M. J., Swarbrick, J., Curtis, S., Gallant, E., Dulhunty, A. F., and O’Bryan, M. K. (2006) J. Biol. Chem., 281, 4156–4163.CrossRefPubMedGoogle Scholar
  24. 24.
    Li, D., Burch, P., Gonzalez, O., Kashork, C., Shaffer, L., Bachinski, L., and Roberts, R. (2000) Biochem. Biophys. Res. Commun., 274, 117–123.CrossRefPubMedGoogle Scholar
  25. 25.
    Maniatis, T., and Tasic, B. (2002) Nature, 418, 236–243.CrossRefPubMedGoogle Scholar
  26. 26.
    Boue, S., Letunic, I., and Bork, P. (2003) Bioessays, 25, 1031–1034.CrossRefPubMedGoogle Scholar
  27. 27.
    Huang, X., Li, J., Lu, L., Xu, M., Xiao, J., Yin, L., Zhu, H., Zhou, Z., and Sha, J. (2005) J. Androl., 26, 189–196.PubMedGoogle Scholar
  28. 28.
    Kratzschmar, J., Haendler, B., Eberspaecher, U., Roosterman, D., Donner, P., and Schleuning, W. (1996) Eur. J. Biochem., 236, 827–836.CrossRefPubMedGoogle Scholar
  29. 29.
    Kido, T., Arata, S., Suzuki, R., Hosono, T., Nakanishi, Y., Miyazaki, J., Saito, I., Kuroki, T., and Shioda, S. (2005) Dev. Growth Differ., 47, 15–24.CrossRefPubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • L. Y. Zhang
    • 1
  • M. Zeng
    • 1
  • P. Chen
    • 1
  • H. Q. Sun
    • 1
  • D. C. Tao
    • 1
  • Y. Q. Liu
    • 1
  • L. Lin
    • 1
  • Y. Yang
    • 1
  • S. Z. Zhang
    • 1
  • Y. X. Ma
    • 1
    Email author
  1. 1.Department of Medical Genetics, State Key Laboratory of Biotherapy, West China HospitalSichuan UniversityChengduP. R. China

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