Identification of messenger RNA substrates for mouse T-STAR
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Using the method of isolation of specific nucleic acids associated with proteins (SNAAP), we have identified 10 candidate target mRNA substrates bound by mT-STAR (mouse T-STAR protein) from testis extract. Among them, our study focused on Fabp9, a gene that is essential for male gametogenesis, and showed that mT-STAR could directly bind to Fabp9 mRNAs. The binding sites are in a short sequence of the coding region and 3′ untranslated region of Fabp9 mRNA. These suggest that mT-STAR can regulate the metabolism and expression of Fabp9. In conclusion, identification of mT-STAR-bound mRNA substrates might help to illustrate the potential spectrum of the process and provide valuable insight into the biological function of this RNA-binding protein in spermatogenesis.
Key wordsFabp9 mRNA substrates mT-STAR SNAAP spermatogenesis
heterogeneous nuclear ribonucleoprotein
mouse T-STAR (testis signal transduction and activation of RNA) protein
RNA binding buffer
specific nucleic acids associated with proteins