Biochemistry (Moscow)

, 74:1203 | Cite as

Primary processes of charge separation in reaction centers of YM210L/FM197Y and YM210L mutants of Rhodobacter sphaeroides

  • A. G. YakovlevEmail author
  • L. G. Vasilieva
  • A. Y. Shkuropatov
  • V. A. Shuvalov


Difference femtosecond absorption spectroscopy with 20-fsec temporal resolution was applied to study a primary stage of charge separation and transfer processes in reaction centers of YM210L and YM210L/FM197Y site-directed mutants of the purple bacterium Rhodobacter sphaeroides at 90 K. Photoexcitation was tuned to the absorption band of the primary electron donor P at 880 nm. Coherent oscillations in the kinetics of stimulated emission of P* excited state at 940 nm and of anion absorption of monomeric bacteriochlorophyll B A at 1020 nm were monitored. The absence of tyrosine YM210 in RCs of both mutants leads to strong slowing of the primary reaction P* → P+B A and to the absence of stabilization of separated charges in the state P+B A . Mutation FM197Y increases effective mass of an acetyl group of pyrrole ring I in the bacteriochlorophyll molecule PB of the double mutant YM210L/FM197Y by a hydrogen bond with OH-TyrM197 group that leads to a decrease in the frequency of coherent nuclear motions from 150 cm−1 in the single mutant YM210L to ∼100 cm−1 in the double mutant. Oscillations with 100–150 cm−1 frequencies in the dynamics of the P* stimulated emission and in the kinetics of the reversible formation of P+B A state of both mutants reflect a motion of the PB molecule relatively to PA in the area of mutual overlapping of their pyrrole rings I. In the double mutant YM210L/FM197Y the oscillations in the P* emission band and the B A absorption band are conserved within a shorter time ∼0.5 psec (1.5 psec in the YM210L mutant), which may be a consequence of an increase in the number of nuclei forming a wave packet by adding a supplementary mass to the dimer P.

Key words

photosynthesis charge separation reaction center wave packet electron transfer 



absorption changes (light minus dark)



BA and BB

monomeric BChl in A- and B-branch, respectively



HA and HB

BPheo in A- and B-branch, respectively


primary electron donor, dimer BChl

PA and PB

BChl molecules forming P

QA and QB

primary and secondary quinone respectively


reaction center






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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • A. G. Yakovlev
    • 1
    Email author
  • L. G. Vasilieva
    • 2
  • A. Y. Shkuropatov
    • 2
  • V. A. Shuvalov
    • 1
    • 2
  1. 1.Department of Photobiophysics, Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Institute of Basic Biological ProblemsRussian Academy of SciencesPushchino, Moscow RegionRussia

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