Biochemistry (Moscow)

, 74:1187 | Cite as

Production of biologically active human myelocytokines in plants

  • A. S. ZverevaEmail author
  • L. E. Petrovskaya
  • A. V. Rodina
  • O. Y. Frolova
  • P. A. Ivanov
  • L. N. Shingarova
  • T. V. Komarova
  • Y. L. Dorokhov
  • D. A. Dolgikh
  • M. P. Kirpichnikov
  • J. G. Atabekov
Accelerated Publication


An effective system for expression of human granulocyte and granulocyte macrophage colony-stimulating factors (hG-CSF and hGM-CSF) in Nicotiana benthamiana plants was developed using viral vector based on tobacco mosaic virus infecting cruciferous plants. The genes of target proteins were cloned into the viral vector driven by actin promoter of Arabidopsis thaliana. The expression vectors were delivered into plant cells by agroinjection. Maximal synthesis rate was detected 5 days after injection and was up to 500 and 300 mg per kg of fresh leaves for hG-CSF and hGM-CSF, respectively. The yield of purified hG-CSF and hGM-CSF was 100 and 50 mg/kg of fresh leaves, respectively. Recombinant plant-made hG-CSF and hGM-CSF stimulated proliferation of murine bone marrow and human erythroleucosis TF-1 cells, respectively, at the same rate as the commercial drugs.

Key words

granulocyte colony-stimulating factor granulocyte macrophage colony-stimulating factor viral vector agroinjection 



colony-forming units in culture


tobacco mosaic virus infecting cruciferous plants


dose necessary for 50% stimulation of proliferation


fetal calf serum


green fluorescent protein


human granulocyte colony-stimulating factor


human granulocyte macrophage colony-stimulating factor




rhG-CSF synthesized in plants


rhGM-CSF synthesized in plants


phenylmethylsulfonyl fluoride


recombinant hG-CSF


recombinant hGM-CSF


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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • A. S. Zvereva
    • 1
    Email author
  • L. E. Petrovskaya
    • 2
  • A. V. Rodina
    • 3
  • O. Y. Frolova
    • 1
  • P. A. Ivanov
    • 1
  • L. N. Shingarova
    • 2
  • T. V. Komarova
    • 4
  • Y. L. Dorokhov
    • 4
  • D. A. Dolgikh
    • 2
  • M. P. Kirpichnikov
    • 1
    • 2
  • J. G. Atabekov
    • 1
  1. 1.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  3. 3.Moscow Institute of Medical EcologyMoscowRussia
  4. 4.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia

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