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Biochemistry (Moscow)

, Volume 74, Issue 10, pp 1080–1087 | Cite as

Reduction of photosystem I reaction center by recombinant DrgA protein in isolated thylakoid membranes of the cyanobacterium Synechocystis sp. PCC 6803

  • I. V. ElanskayaEmail author
  • V. A. Toporova
  • V. G. Grivennikova
  • E. M. Muronets
  • E. P. Lukashev
  • K. N. Timofeev
Article
  • 64 Downloads

Abstract

To study the function of soluble NAD(P)H:quinone oxidoreductase of the cyanobacterium Synechocystis sp. PCC 6803 encoded by drgA gene, recombinant DrgA protein carrying 12 histidine residues on the C-terminal end was expressed in Escherichia coli and purified. Recombinant DrgA is a flavoprotein that exhibits quinone reductase and nitroreductase activities with NAD(P)H as the electron donor. Using EPR spectroscopy, it was demonstrated that addition of recombinant DrgA protein and NADPH to DCMU-treated isolated thylakoid membranes of the cyanobacterium increased the dark rereduction rate of the photosystem I reaction center (P700+). Thus, DrgA can participate in electron transfer from NADPH to the electron transport chain of the Synechocystis sp. PCC 6803 thylakoid membrane.

Key words

cyanobacteria drgA gene NAD(P)H:quinone oxidoreductase cyclic electron transport around photosystem I EPR spectroscopy 

Abbreviations

DCMU

3-(3,4-dichlorophenyl)-1,1-dimethyl-urea

DCPIP

2,6-dichlorophenol indophenol; decylplasto-quinone, 2,3-dimethyl-6-decyl-1,4-benzoquinone; dinoseb, 2-sec-butyl-4,6-dinitrophenol; duroquinone, tetramethyl-1,4-benzoquinone

ETC

electron transport chain

Fd

ferredoxin

FMN

flavin mononucleotide

FNR

ferredoxin:NADP+-oxidoreductase

IPTG

isopropyl-β-D-thiogalactopyranoside

PCR

polymerase chain reaction

PMSF

phenylmethylsulfonyl fluoride

PQ

plastoquinone

PS

photosystem

Q0

2,3-dimethoxy-5-methyl-1,4-benzoquinone (ubiquinone Q0)

SOD

superoxide dismutase

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • I. V. Elanskaya
    • 1
    Email author
  • V. A. Toporova
    • 2
  • V. G. Grivennikova
    • 1
  • E. M. Muronets
    • 1
  • E. P. Lukashev
    • 1
  • K. N. Timofeev
    • 1
  1. 1.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia

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