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Biochemistry (Moscow)

, Volume 74, Issue 9, pp 971–978 | Cite as

Structural-functional characteristics of a new bioregulator isolated from bovine pigmented epithelium tissue

  • V. P. YamskovaEmail author
  • V. S. Skripnikova
  • A. A. Molyavka
  • A. P. Il’ina
  • M. S. Krasnov
  • D. V. Margasyuk
  • A. V. Borisenko
  • B. B. Berezin
  • E. S. Kuznetsova
  • A. K. Buryak
  • I. A. Yamskov
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Abstract

A new bioregulator operating in ultralow doses corresponding to 10−17 mg/ml has been isolated from tissue of pigmented epithelium of bovine eyes. It has been established that the functional basis of this bioregulator is a complex of a low molecular weight regulatory peptide (4372 Da) and a modulator consisting of a mixture of proteins with molecular weights of 14.980–66.283 kDa. It has been shown that the regulatory peptide is responsible for membranotropic activity of the bioregulator, and the modulator proteins are responsible for biological action in ultralow doses. The data demonstrate an interrelation between nanocondition of the bioregulator and its ability to show activity in ultralow doses.

Key words

bioregulators regulatory proteins nanoparticles ultralow doses 

Abbreviations

AFM

atomic-force microscopy

PE

pigmented epithelium

RP

regulatory proteins

TFA

trifluoroacetic acid

ULD

ultralow doses

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • V. P. Yamskova
    • 1
    Email author
  • V. S. Skripnikova
    • 2
  • A. A. Molyavka
    • 2
  • A. P. Il’ina
    • 2
  • M. S. Krasnov
    • 1
  • D. V. Margasyuk
    • 2
  • A. V. Borisenko
    • 2
  • B. B. Berezin
    • 2
  • E. S. Kuznetsova
    • 3
  • A. K. Buryak
    • 3
  • I. A. Yamskov
    • 2
  1. 1.Koltzov Institute of Developmental BiologyRussian Academy of SciencesMoscowRussia
  2. 2.Nesmeyanov Institute of Organoelemental CompoundsRussian Academy of SciencesMoscowRussia
  3. 3.Frumkin Institute of Physical Chemistry and ElectrochemistryRussian Academy of SciencesMoscowRussia

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