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Biochemistry (Moscow)

, Volume 74, Issue 6, pp 655–662 | Cite as

Enzymological properties of endo-(1–4)-β-glucanase Eg12p of Penicillium canescens and characteristics of structural gene egl2

  • A. M. Chulkin
  • D. S. LoginovEmail author
  • E. A. Vavilova
  • A. R. Abyanova
  • I. N. Zorov
  • S. A. Kurzeev
  • O. V. Koroleva
  • S. V. Benevolenskii
Article

Abstract

Gene egl2 of secreted endo-(1–4)-β-glucanase of glycosyl hydrolase family 5 of the mycelial fungus Penicillium canescens was cloned. The gene was expressed in P. canescens under control of a strong promoter of the bgaS gene encoding β-galactosidase of P. canescens, and endoglucanase producing strains were obtained. Chromatographically purified recombinant 48 kDa protein had pH and temperature optima 3.4 and 60°C, respectively, exhibited specific activity of 33 IU, and had K m and V max in CM-cellulose hydrolysis of 10.28 g/liter and 0.26 μmol/sec per mg, respectively.

Key words

endoglucanase mycelial fungus Penicillium canescens gene 

Abbreviations

CMC

CM-cellulose

DSC

differential scanning calorimetry

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • A. M. Chulkin
    • 1
  • D. S. Loginov
    • 1
    Email author
  • E. A. Vavilova
    • 1
  • A. R. Abyanova
    • 1
  • I. N. Zorov
    • 1
    • 2
  • S. A. Kurzeev
    • 1
    • 2
  • O. V. Koroleva
    • 1
  • S. V. Benevolenskii
    • 1
  1. 1.Bach Institute of BiochemistryRussian Academy of SciencesMoscowRussia
  2. 2.Lomonosov Moscow State UniversityMoscowRussia

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