Advertisement

Biochemistry (Moscow)

, Volume 74, Issue 6, pp 633–642 | Cite as

Resistance of α-crystallin quaternary structure to UV irradiation

  • A. V. KrivandinEmail author
  • K. O. Muranov
  • F. Yu. Yakovlev
  • N. B. Poliansky
  • L. A. Wasserman
  • M. A. Ostrovsky
Article

Abstract

The damaging effect of UV radiation (λ > 260 nm) on bovine α-crystallin in solution was studied by small-angle X-ray scattering, gel permeation chromatography, electrophoresis, absorption and fluorescence spectroscopy, and differential scanning calorimetry. The results obtained show that damage to even a large number of subunits within an α-crystallin oligomer does not cause significant rearrangement of its quaternary structure, aggregation of oligomers, or the loss of their solubility. Due to the high resistance of its quaternary structure, α-crystallin is able to prevent aggregation of destabilized proteins (especially of γ- and β-crystallins) and so to maintain lens transparency throughout the life of an animal (the chaperone-like function of α-crystallin).

Key words

α-crystallin quaternary structure UV radiation small-angle X-ray scattering 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Horwitz, J. (2003) Exp. Eye Res., 76, 145–153.PubMedCrossRefGoogle Scholar
  2. 2.
    Vanhoudt, J., et al. (2000) Biochemistry, 39, 4483.PubMedCrossRefGoogle Scholar
  3. 3.
    Siezen, R. J., and Berger, H. (1978) Eur. J. Biochem., 91, 397–405.PubMedCrossRefGoogle Scholar
  4. 4.
    Tardieu, A., Laporte, D., Lichino, P., Krop, B., and Delae, M. (1986) J. Mol. Biol., 192, 711–724.PubMedCrossRefGoogle Scholar
  5. 5.
    Groenen, P. J. T. A., Merck, K. B., de Jong, W. W., and Bloemendal, H. (1994) Eur. J. Biochem., 225, 1–19.PubMedCrossRefGoogle Scholar
  6. 6.
    Groth-Vasselli, B., Kumosinski, T. F., and Farnsworth, P. N. (1995) Exp. Eye Res., 61, 249–253.PubMedCrossRefGoogle Scholar
  7. 7.
    Krivandin, A. V., Muranov, K. O., and Ostrovsky, M. A. (2004) Mol. Biol. (Moscow), 38, 532–546.Google Scholar
  8. 8.
    Horwitz, J. (1992) Proc. Natl. Acad. Sci. USA, 89, 10449–10453.PubMedCrossRefGoogle Scholar
  9. 9.
    Wang, K., and Spector, A. (1994) J. Biol. Chem., 269, 13601–13608.PubMedGoogle Scholar
  10. 10.
    Rao, P. V., Huang, Q., Horwitz, J., and Zigler, J. S. (1995) Biochim. Biophys. Acta, 1245, 439–447.PubMedGoogle Scholar
  11. 11.
    Borkman, R. F., Knight, G., and Obi, B. (1996) Exp. Eye Res., 62, 141–148.PubMedCrossRefGoogle Scholar
  12. 12.
    Krivandin, A. V., Muranov, K. O., Poturaeva, I. D., Polyanskii, N. B., and Ostrovskii, M. A. (2006) Doklady Ros. Akad. Nauk, 409, 550–554.Google Scholar
  13. 13.
    Abgar, S., Yevlampieva, N., Aerts, T., Vanhoudt, J., and Clauwaert, J. (2000) Biochem. Biophys. Res. Commun., 276, 619–625.PubMedCrossRefGoogle Scholar
  14. 14.
    Das, K. P., and Surewicz, W. K. (1995) FEBS Lett., 369, 321–325.PubMedCrossRefGoogle Scholar
  15. 15.
    Boyle, D., and Takemoto, L. (1994) Exp. Eye Res., 58, 9–16.PubMedCrossRefGoogle Scholar
  16. 16.
    Neal, R. E., Purdie, J. L., Hirst, L. W., and Green, A. C. (2003) Epidemiology, 14, 707–712.CrossRefGoogle Scholar
  17. 17.
    Korkhmazyan, M. M., Fedorovich, I. B., and Ostrovsky, M. A. (1983) Biofizika, 28, 966–971.Google Scholar
  18. 18.
    Krivandin, A. V., Lvov, Yu. M., Ostrovskii, M. A., Fedorovich, I. B., and Feigin, L. A. (1989) Exp. Eye Res., 49, 853–859.PubMedCrossRefGoogle Scholar
  19. 19.
    Elchaninov, V. V., and Fedorovich, I. B. (1989) Biofizika, 34, 758–762.Google Scholar
  20. 20.
    Hott, J. L., and Borkman, R. F. (1993) Photochem. Photobiol., 57, 312–317.PubMedCrossRefGoogle Scholar
  21. 21.
    Ostrovsky, M. A., Sergeev, Y. V., Atkinson, D. B., Soustov, L. V., and Hejtmancik, J. F. (2002) Mol. Vis., 8, 72–78.PubMedGoogle Scholar
  22. 22.
    Andley, U. P., Sutherland, P., Liang, J. N., and Chakrabarti, B. (1984) Photochem. Photobiol., 40, 343–349.PubMedCrossRefGoogle Scholar
  23. 23.
    Borkman, R. F., and McLaughlin, J. (1995) Photochem. Photobiol., 62, 1046–1051.PubMedCrossRefGoogle Scholar
  24. 24.
    Ellozy, A. R., Ceger, P., Wang, R. H., and Dillon, J. (1996) Photochem. Photobiol., 64, 344–348.PubMedCrossRefGoogle Scholar
  25. 25.
    Finley, E. L., Busman, M., Dillon, J., Crouch, R. K., and Schey, K. L. (1997) Photochem. Photobiol., 66, 635–641.PubMedCrossRefGoogle Scholar
  26. 26.
    Lin, S. Y., Ho, C. J., and Li, M. J. (1999) J. Photochem. Photobiol. B., 49, 29–34.PubMedCrossRefGoogle Scholar
  27. 27.
    Ervin, L. A., Dillon, J., and Gaillard, E. R. (2001) Photochem. Photobiol., 73, 685–691.PubMedCrossRefGoogle Scholar
  28. 28.
    Fujii, N., Uchida, H., and Saito, T. (2004) Mol. Vis., 10, 814–820.PubMedGoogle Scholar
  29. 29.
    Weinreb, O., van Boekel, M. A., Dovrat, A., and Bloemendal, H. (2000) Invest. Ophthalmol. Vis. Sci., 41, 191–198.PubMedGoogle Scholar
  30. 30.
    Mandal, K., Bose, S. K., and Chakrabarti, B. (1986) Photochem. Photobiol., 43, 515–523.PubMedCrossRefGoogle Scholar
  31. 31.
    Vekshin, N. L., and Sukharev, V. I. (2005) Biofizika, 50, 236–242.PubMedGoogle Scholar
  32. 32.
    Feigin, L. A., and Svergun, D. I. (1987) Structure Analysis by Small-Angle X-Ray and Neutron Scattering, Plenum Press, N.Y.Google Scholar
  33. 33.
    Laemmli, U. K. (1970) Nature, 227, 680–685.PubMedCrossRefGoogle Scholar
  34. 34.
    Vasil’ev, S. E., Donets, D. E., Zanevskii, Yu. V., Ivanov, A. B., Smykov, L. P., Cheremukhina, G. A., and Chernenko, S. P. (1995) Pribory Tekhn. Eksp., 2, 172–177.Google Scholar
  35. 35.
    Konarev, P. V., Volkov, V. V., Sokolova, A. V., Koch, M. H. J., and Svergun, D. I. (2003) J. Appl. Cryst., 36, 1277–1282.CrossRefGoogle Scholar
  36. 36.
    Svergun, D. I. (1992) J. Appl. Cryst., 25, 495–503.CrossRefGoogle Scholar
  37. 37.
    Shchedrin, B. M., and Feigin, L. A. (1966) Kristallografiya, 11, 159–163.Google Scholar
  38. 38.
    Peters, T., Jr. (1997) All About Albumin-Biochemistry, Genetics, and Medical Applications, Academic Press, San Diego, CA.Google Scholar
  39. 39.
    Pirie, A. (1971) Biochem. J., 125, 203–208.PubMedGoogle Scholar
  40. 40.
    Walsh, M. T., Sen, A. C., and Chakrabarti, B. (1991) J. Biol. Chem., 266, 20079–20084.PubMedGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • A. V. Krivandin
    • 1
    Email author
  • K. O. Muranov
    • 1
  • F. Yu. Yakovlev
    • 1
  • N. B. Poliansky
    • 1
  • L. A. Wasserman
    • 1
  • M. A. Ostrovsky
    • 1
  1. 1.Emanuel Institute of Biochemical PhysicsRussian Academy of SciencesMoscowRussia

Personalised recommendations