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Biochemistry (Moscow)

, Volume 74, Issue 5, pp 578–587 | Cite as

Reversibility of nucleoside diphosphate kinase solubilization from the surface of the outer mitochondrial membrane

  • T. Yu. LipskayaEmail author
  • V. V. Voinova
Article

Abstract

It was found that in medium with low ionic strength nucleoside diphosphate kinase (NDPK) solubilization from the outer membrane of liver mitochondria could be partially reversed by the addition of 3.3 mM MgCl2. Complete rebinding of the enzyme after the addition of MgCl2 was observed when the mitochondrial washing and storage medium contained leupeptin, an inhibitor of cathepsins. It was demonstrated that leupeptin and another inhibitor of cysteine proteinases, E-64, do not influence the rate of NDPK solubilization as well as its solubilized and membrane-associated activity. We conclude that NDPK becomes sensitive to proteolysis only after its solubilization; proteolysis does not affect the part of the enzyme molecule that is responsible for catalysis. After solubilization of NDPK in the absence of leupeptin, cathepsins damage sites of its binding on the membranes. The rate of the enzyme solubilization is dependent on the pH of the storage medium (pH 6.0–8.0); it decreases with increase in pH. It was shown that in the medium with high ionic strength, MgCl2 does not reverse pH-dependent NDPK solubilization, but solubilization could be reversed by increase in medium pH in the presence of E-64 and BSA. The physiological importance of these results is discussed.

Key words

nucleoside diphosphate kinase mitochondria outer compartment solubilization liver 

Abbreviations

AP5A

p1p5-di(adenosine-5′)pentaphosphate

DMO

5,5-dimethyl-2,4-oxazolidinedione

E-64

L-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane

HK

hexokinase

leupeptin

acetyl-leucyl-leucyl-arginal

NDPK

nucleoside diphosphate kinase

omNDPK

outer mitochondrial membrane bound NDPK

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  1. 1.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia

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