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Biochemistry (Moscow)

, Volume 74, Issue 5, pp 543–548 | Cite as

Anionic polymers of the cell wall of Bacillus subtilis subsp. subtilis VKM B-501T

  • A. S. Shashkov
  • N. V. PotekhinaEmail author
  • S. N. Senchenkova
  • E. B. Kudryashova
Article

Abstract

Teichoic acid and disaccharide-1-phosphate polymer were identified in the cell walls of Bacillus subtilis subsp. subtilis VKM B-501T. The teichoic acid represents 1,3-poly(glycerol phosphate) 80% substituted by α-D-glucopyranose residues at O-2 of glycerol. The linear repeating unit of disaccharide-1-phosphate polymer contains the residues of β-D-glucopyranose, N-acetyl-α-D-galactosamine, and phosphate and has the following structure: -6)-β-D-Glcp-(1→3)-α-D-GalpNAc-(1-P-. The structures of two anionic polymers were determined by chemical and NMR-spectroscopic methods. The 1H- and 13C-NMR spectral data on disaccharide-1-phosphate polymer are presented for the first time.

Key words

Bacillus subtilis cell wall teichoic acid disaccharide-1-phosphate polymer anionic polysaccharides NMR spectroscopy 

Abbreviations

PE1

phosphoric ester

TA

teichoic acids

TSP

sodium salt of 3-(trimethylsilyl)-3,3,2,2-tetradeuteropropionic acid

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • A. S. Shashkov
    • 1
  • N. V. Potekhina
    • 2
    Email author
  • S. N. Senchenkova
    • 1
  • E. B. Kudryashova
    • 3
  1. 1.Zelinsky Institute of Organic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Faculty of BiologyLomonosov Moscow State UniversityMoscowRussia
  3. 3.All-Russian Collection of Microorganisms (VKM), Skryabin Institute of Biochemistry and Physiology of MicroorganismsRussian Academy of SciencesPushchino, Moscow RegionRussia

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