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Biochemistry (Moscow)

, Volume 74, Issue 5, pp 533–542 | Cite as

Polysaccharides of basidiomycetes. Alkali-soluble polysaccharides from the mycelium of white rot fungus Ganoderma lucidum (Curt.: Fr.) P. Karst

  • M. S. Evsenko
  • A. S. Shashkov
  • A. V. Avtonomova
  • L. M. Krasnopolskaya
  • A. I. UsovEmail author
Article

Abstract

Two polysaccharides were isolated from submergedly cultured mycelium of the basidiomycete Ganoderma lucidum by extraction with alkali followed by fractionation with Fehling reagent. The polysaccharides were shown to be a linear (1→3)-α-D-glucan and a highly branched xylomannan containing a backbone built up of (1→3)-linked α-D-mannopyranose residues, the majority of which are substituted at O-4 by single β-D-xylopyranose residues or by disaccharide fragments β-D-Manp-(1→3)-β-D-Xylp-(1→. Polysaccharide structures were elucidated by NMR spectroscopy in combination with methylation analysis and periodate oxidation. An interesting feature of the xylomannan is the simultaneous presence of α-D-mannopyranose and β-D-mannopyranose residues, the first forming the backbone, and the second being the non-reducing terminal units of disaccharide side chains.

Key words

Basidiomycetes white rot fungi Ganoderma lucidum polysaccharides (1→3)-α-D-glucan branched xylomannan NMR spectroscopy 

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • M. S. Evsenko
    • 1
    • 2
  • A. S. Shashkov
    • 1
  • A. V. Avtonomova
    • 2
  • L. M. Krasnopolskaya
    • 2
  • A. I. Usov
    • 1
    Email author
  1. 1.Zelinsky Institute of Organic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Gause Institute of New AntibioticsRussian Academy of Medical SciencesMoscowRussia

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