Biochemistry (Moscow)

, Volume 74, Issue 5, pp 509–517 | Cite as

Manganese-dependent carboanhydrase activity of photosystem II proteins

  • A. V. ShitovEmail author
  • O. V. Pobeguts
  • T. N. Smolova
  • S. I. Allakhverdiev
  • V. V. Klimov


Four sources of carbonic anhydrase (CA) activity in submembrane preparations of photosystem II (PS II) isolated from pea leaves were examined. Three of them belong to the hydrophilic proteins of the oxygen-evolving complex of PS II with molecular mass 33 kDa (protein PsbO), 24 kDa (protein PsbP), and 18 kDa (protein PsbQ). The fourth source of CA activity is associated with a pigment-protein complex of PS II after removing three hydrophilic proteins by salt treatment. Except for protein PsbQ, the CA activity of all these proteins depends on the presence of Mn2+: the purified protein PsbO did not show CA activity before adding Mn2+ into the medium (concentration of Mn2+ required for 50% effect, EC50, was 670 μM); CA activity of protein mixture composed of PsbP and PsbQ increased more than 5-fold upon adding Mn2+ (EC50 was 45 μM). CA activity of purified protein PsbP increased 2-fold in the presence of 200 μM Mn2+. As indicated for the mixture of two proteins (PsbP and PsbQ), Mg2+, Ca2+, and Zn2+, in contrast to Mn2+, suppressed CA activity (both initial and Mn2+-induced activity). Since the found sources of CA activity demonstrated properties different from ones of typical CA (need for Mn2+, insensitivity or low sensitivity to acetazolamide or ethoxyzolamide) and such CA activity was found only among PS II proteins, we cannot exclude that they belong to the type of Mn-dependent CA associated with PS II.

Key words

photosystem II carbonic anhydrase activity oxygen-evolving complex hydrophilic proteins of PS II-PsbO PsbP PsbQ 





bovine serum albumin


carbonic anhydrase




concentration of a compound necessary to produce 50% of its maximal effect




light-harvesting complex 2


photosystem I


photosystem II


reaction center


water oxidizing complex


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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • A. V. Shitov
    • 1
    Email author
  • O. V. Pobeguts
    • 1
  • T. N. Smolova
    • 1
  • S. I. Allakhverdiev
    • 1
  • V. V. Klimov
    • 1
  1. 1.Institute of Basic Biological ProblemsRussian Academy of SciencesPushchino, Moscow RegionRussia

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