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Biochemistry (Moscow)

, Volume 74, Issue 4, pp 438–444 | Cite as

Electrochemistry of cytochromes P450: Analysis of current-voltage characteristics of electrodes with immobilized cytochromes P450 for the screening of substrates and inhibitors

  • V. V. ShumyantsevaEmail author
  • T. V. Bulko
  • G. P. Kuznetsova
  • N. F. Samenkova
  • A. I. Archakov
Article

Abstract

In the current study, an approach to elucidating the substrate specificity of cytochromes P450 based on the analysis of current-voltage characteristics of voltammograms and amperograms is proposed. Data on the electrochemical behavior of bioelectrodes with immobilized cytochromes P450 2B4, 1A2, 3A4, 11A1 (P450scc), and 51b1 (Mycobacterium tuberculosis sterol 14α-demethylase or CYP51 MT) in the presence of typical substrates and inhibitors for these hemoprotein forms are reported. Immobilization of the enzymes was accomplished by using graphite screen-printed electrodes modified with gold nanoparticles and with the synthetic membrane-like compound didodecyldimethylammonium bromide. The method of electro-analysis can be applied to the search of potential substrates and inhibitors of cytochromes P450 and to creation of multichannel electrochemical plates (chips, panels) with immobilized cytochromes P450.

Key words

cytochrome P450 colloidal gold nanoparticles bioelectrochemistry drug metabolism 

Abbreviations

Bz

benzphetamine

CV

cyclic voltammetry

DDAB

didodecyldimethylammonium bromide

DPV

differential pulse voltammetry

Epa and Epc

potentials of anode reduction and cathode oxidation peaks, respectively

IPA

intermittent pulse amperometry

SWV

square wave voltammetry

Ts

testosterone

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • V. V. Shumyantseva
    • 1
    Email author
  • T. V. Bulko
    • 1
  • G. P. Kuznetsova
    • 1
  • N. F. Samenkova
    • 1
  • A. I. Archakov
    • 1
  1. 1.Institute of Biomedical ChemistryRussian Academy of Medical SciencesMoscowRussia

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