Biochemistry (Moscow)

, Volume 74, Issue 4, pp 430–437 | Cite as

Analysis of mitochondrial DNA somatic mutations in OXYS and Wistar strain rats

  • U. N. Rotskaya
  • I. B. Rogozin
  • E. A. Vasyunina
  • N. G. Kolosova
  • B. A. Malyarchuk
  • G. A. Nevinsky
  • O. I. SinitsynaEmail author


Rats of the OXYS strain are sensitive to oxidative stress and serve as a biological model of premature aging. We have compared spectra of somatic mutations in a control region of mtDNA from the liver of the OXYS rat strain and of Wistar rats as a control. The majority of nucleotide substitutions in the mutation spectra were represented by transitions: 94 and 97% in the OXYS and Wistar rats, respectively. It was shown that 40% of somatic mutations in the control region of mtDNA from Wistar rats were significantly consistent with the model of dislocation mutagenesis. No statistical support for this model was found for mutations in the control region of mtDNA from OXYS rats. The mutation frequency in the ETAS section was higher in the OXYS strain rats than in Wistar rats. These results suggest different mechanisms of mutagenesis in the two rat strains under study.

Key words

OXYS rats Wistar rats somatic mutations mtDNA liver 



mitochondrial DNA



Pol γ

DNA polymerase γ


reactive oxygen species


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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • U. N. Rotskaya
    • 1
  • I. B. Rogozin
    • 1
  • E. A. Vasyunina
    • 1
  • N. G. Kolosova
    • 1
  • B. A. Malyarchuk
    • 2
  • G. A. Nevinsky
    • 1
    • 3
  • O. I. Sinitsyna
    • 1
    Email author
  1. 1.Institute of Cytology and GeneticsSiberian Division of the Russian Academy of SciencesNovosibirskRussia
  2. 2.Institute of Biological Problems of the NorthFar-Eastern Division of the Russian Academy of SciencesMagadanRussia
  3. 3.Institute of Chemical Biology and Fundamental MedicineSiberian Division of the Russian Academy of SciencesNovosibirskRussia

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