Advertisement

Biochemistry (Moscow)

, Volume 74, Issue 4, pp 430–437 | Cite as

Analysis of mitochondrial DNA somatic mutations in OXYS and Wistar strain rats

  • U. N. Rotskaya
  • I. B. Rogozin
  • E. A. Vasyunina
  • N. G. Kolosova
  • B. A. Malyarchuk
  • G. A. Nevinsky
  • O. I. SinitsynaEmail author
Article

Abstract

Rats of the OXYS strain are sensitive to oxidative stress and serve as a biological model of premature aging. We have compared spectra of somatic mutations in a control region of mtDNA from the liver of the OXYS rat strain and of Wistar rats as a control. The majority of nucleotide substitutions in the mutation spectra were represented by transitions: 94 and 97% in the OXYS and Wistar rats, respectively. It was shown that 40% of somatic mutations in the control region of mtDNA from Wistar rats were significantly consistent with the model of dislocation mutagenesis. No statistical support for this model was found for mutations in the control region of mtDNA from OXYS rats. The mutation frequency in the ETAS section was higher in the OXYS strain rats than in Wistar rats. These results suggest different mechanisms of mutagenesis in the two rat strains under study.

Key words

OXYS rats Wistar rats somatic mutations mtDNA liver 

Abbreviations

mtDNA

mitochondrial DNA

8-oxoG

8-oxoguanine

Pol γ

DNA polymerase γ

ROS

reactive oxygen species

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Anderson, S., Bankier, A. T., Barrel, B. G., de Bruijn, M. H. L., Coulson, A. R., Drouin, J., Eperon, I. C., Nierlich, D. R., Roe, B. A., Sanger, F., Schreier, P. H., Smith, A. J. M., Staden, R., and Young, I. G. (1981) Nature, 290, 457–467.PubMedCrossRefGoogle Scholar
  2. 2.
    Chomyn, A., and Attardi, G. (2003) Biochem. Biophys. Res. Commun., 304, 519–529.PubMedCrossRefGoogle Scholar
  3. 3.
    Kujoth, G. C., Bradshaw, P. C., Haroon, S., and Prolla, T. A. (2007) PLoS Genet., 3, 24.CrossRefGoogle Scholar
  4. 4.
    Michikawa, Y., Mazzucchelli, F., Bresolin, N., Scarlato, G., and Attardi, G. (1999) Science, 286, 774–779.PubMedCrossRefGoogle Scholar
  5. 5.
    Khrapko, K., Kraytsberg, Y., de Grey, A. D., Vijg, J., and Schon, E. A. (2006) Aging Cell, 5, 279–282.PubMedCrossRefGoogle Scholar
  6. 6.
    Kujoth, G. C., Hiona, A., Pugh, T. D., Someya, S., Panzer, K., Wohlgemuth, S. E., Hofer, T., Seo, A. Y., Sullivan, R., Jobling, W. A., Morrow, J. D., van Remmen, H., Sedivy, J. M., Yamasoba, T., Tanokura, M., Weindruch, R., Leeuwenburgh, C., and Prolla, T. A. (2005) Science, 309, 481–484.PubMedCrossRefGoogle Scholar
  7. 7.
    Vermulst, M., Bielas, J. H., Kujoth, G. C., Ladiges, W. C., Rabinovitch, P. S., Prolla, T. A., and Loeb, L. A. (2007) Nat. Genet., 39, 540–543.PubMedCrossRefGoogle Scholar
  8. 8.
    Khrapko, K., and Vijg, J. (2007) Nat. Genet., 39, 445–446.PubMedCrossRefGoogle Scholar
  9. 9.
    Bobko, A. A., Sergeeva, S. V., Bagryanskaya, E. G., Markel, A. L., Khramtsov, V. V., Reznikov, V. A., and Kolosova, N. G. (2005) Biochem. Biophys. Res. Commun., 330, 367–370.PubMedCrossRefGoogle Scholar
  10. 10.
    Kolosova, N. G., Lebedev, P. A., Aidagulova, S. V., and Morozkova, T. S. (2003) Byul. Eksp. Biol. Med., 136, 415–419.CrossRefGoogle Scholar
  11. 11.
    Salganik, R. I., Solovyova, N. A., Dikalov, S. I., Grishaeva, O. N., Semenova, L. A., and Popovsky, A. V. (1994) Biochem. Biophys. Res. Commun., 199, 726–733.PubMedCrossRefGoogle Scholar
  12. 12.
    Shabalina, I. G., Kolosova, N. G., Grishanova, A. Yu., Solovyova, N. A., Solovyov, V. N., and Salganik, R. I. (1995) Biochemistry (Moscow), 60, 1563–1568.Google Scholar
  13. 13.
    Ishchenko, A., Sinitsyna, O. I., Krysanova, Z. S., Vasyunina, E. A., Saparbaev, M. N., Sidorkina, O., and Nevinsky, G. A. (2003) Med. Sci. Monitor., 9, 16–24.Google Scholar
  14. 14.
    Kemeleva, E. A., Sinitsyna, O. I., Kolosova, N. G., Vasyunina, E. A., Zharkov, D. O., Conlon, K. A., Berrios, M., and Nevinsky, G. A. (2006) Mutat. Res., 599, 88–97.PubMedGoogle Scholar
  15. 15.
    Kemeleva, E. A., Sinitsyna, O. I., Conlon, K. A., Berrios, M., Kolosova, N. G., Zharkov, D. O., Vasyunina, E. A., and Nevinsky, G. A. (2006) Biochemistry (Moscow), 71, 612–618.CrossRefGoogle Scholar
  16. 16.
    Sinitsina, O. I., Krysanova, Z. S., Ishchenko, A., Dikalova, A. E., Stolyarov, S., Kolosova, N. G., Vasyunina, E. A., and Nevinsky, G. A. (2006) J. Cell. Mol. Med., 10, 206–215.CrossRefGoogle Scholar
  17. 17.
    Shabalina, I. G., Shalbueva, N. I., Dikalova, A. E., Sinitsyna, O. I., Krysanova, Zh. S., and Kolosova, N. G. (2001) Klin. Eksp. Farmakol., 60, 34–36.Google Scholar
  18. 18.
    Kolosova, N. G., Shcheglova, T. V., Sergeeva, S. V., and Loskutova, L. V. (2006) Neurobiol. Aging, 27, 1289–1297.PubMedCrossRefGoogle Scholar
  19. 19.
    Kolosova, N. G., Shcheglova, T. V., Amstislavskaya, T. G., and Loskutova, L. V. (2003) Byul. Eksp. Biol., 135, 696–699.Google Scholar
  20. 20.
    Kolosova, N. G., Grishanova, A. Yu., Krysanova, Zh. S., Zueva, T. V., and Sinitsina, O. I. (2004) Biomed. Khim., 50, 73–78.PubMedGoogle Scholar
  21. 21.
    Kolosova, N. G., Lebedev, P. A., and Dikalova, A. E. (2004) Byul. Eksp. Biol., 137, 249–251.CrossRefGoogle Scholar
  22. 22.
    Excoffier, L., and Yang, Z. (1999) Mol. Biol. Evol., 16, 1357–1368.PubMedGoogle Scholar
  23. 23.
    Heyer, E., Zietkiewicz, E., Rochowski, A., Yotova, V., Puymirat, J., and Labuda, D. (2001) Am. J. Hum. Genet., 69, 1113–1126.PubMedCrossRefGoogle Scholar
  24. 24.
    Meyer, S., Weiss, G., and von Haeseler, A. (1999) Genetics, 152, 1103–1110.PubMedGoogle Scholar
  25. 25.
    Wakeley, J. (1993) J. Mol. Evol., 37, 613–623.PubMedCrossRefGoogle Scholar
  26. 26.
    Malyarchuk, B. A., and Rogozin, I. B. (2004) Ann. Hum. Genet., 68, 324–339.PubMedCrossRefGoogle Scholar
  27. 27.
    Malyarchuk, B. A., Rogozin, I. B., Berikov, V. B., and Derenko, M. V. (2002) Hum. Genet., 111, 46–53.PubMedCrossRefGoogle Scholar
  28. 28.
    Saccone, C., Pesole, G., and Sbisa, E. (1991) J. Mol. Evol., 33, 83–91.PubMedCrossRefGoogle Scholar
  29. 29.
    Schlick, N. E., Jensen-Seaman, M. I., Orlebeke, K., Kwitek, A. E., Jacob, H. J., and Lazar, J. (2006) Am. J. Physiol. Cell Physiol., 291, 1183–1192.CrossRefGoogle Scholar
  30. 30.
    Miller, D. G. (1972) in Experiments in Molecular Genetics (Alikhanyan, S. I., ed.) [Russian translation], Mir, Moscow.Google Scholar
  31. 31.
    Corpet, F. (1988) Nucleic Acids Res., 16, 10881–10890.PubMedCrossRefGoogle Scholar
  32. 32.
    Glazko, G. V., Milanesi, L., and Rogozin, I. B. (1998) J. Theor. Biol., 192, 475.PubMedCrossRefGoogle Scholar
  33. 33.
    Rogosin, I. B., Kondrashov, F. A., and Glazko, G. V. (2001) Hum. Mutat., 17, 83.CrossRefGoogle Scholar
  34. 34.
    Tamura, K. (2000) Gene, 259, 189–197.PubMedCrossRefGoogle Scholar
  35. 35.
    Vigilant, L., Stoneking, M., Harpending, H., Hawkes, K., and Wilson, A. C. (1991) Science, 253, 1503–1507.PubMedCrossRefGoogle Scholar
  36. 36.
    Rogozin, I. B., and Pavlov, Y. I. (2003) Mutat. Res., 544, 65–85.PubMedCrossRefGoogle Scholar
  37. 37.
    Clayton, D. A. (1991) Annu. Rev. Cell Biol., 7, 453–478.PubMedCrossRefGoogle Scholar
  38. 38.
    Sbisa, E., Tanzariello, F., Reyes, A., Pesole, G., and Saccone, C. (1997) Gene, 205, 125–140.PubMedCrossRefGoogle Scholar
  39. 39.
    Larizza, A., Pesole, G., Reyes, A., Sbisa, E., and Saccone, C. (2002) J. Mol. Evol., 54, 145–155.PubMedCrossRefGoogle Scholar
  40. 40.
    Kunkel, T. A., and Soni, A. (1988) J. Biol. Chem., 263, 14784–14789.PubMedGoogle Scholar
  41. 41.
    Rogozin, I. B., and Kolchanov, N. A. (1995) J. Cell. Biochem., 21, 345.Google Scholar
  42. 42.
    Marcelino, L. A., and Thilly, W. G. (1999) Mutat. Res., 434, 177–203.PubMedGoogle Scholar
  43. 43.
    Bebenek, K., and Kunkel, T. A. (2000) Cold Spring Harb. Symp. Quant. Biol., 65, 81–91.PubMedCrossRefGoogle Scholar
  44. 44.
    Larsson, N. G., Wang, J., Wilhelmsson, H., Oldfors, A., Rustin, P., Lewandoski, M., Barsh, G. S., and Clayton, D. A. (1998) Nature Genet., 18, 231–236.PubMedCrossRefGoogle Scholar
  45. 45.
    Jagadeessan, N., Sinitsyna, O. I., Vasyunina, E. A., Nevinsky, G. A., Laval, J., and Bartsch, H. (2005) Biochem. Biophys. Res. Commun., 336, 478–482.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • U. N. Rotskaya
    • 1
  • I. B. Rogozin
    • 1
  • E. A. Vasyunina
    • 1
  • N. G. Kolosova
    • 1
  • B. A. Malyarchuk
    • 2
  • G. A. Nevinsky
    • 1
    • 3
  • O. I. Sinitsyna
    • 1
    Email author
  1. 1.Institute of Cytology and GeneticsSiberian Division of the Russian Academy of SciencesNovosibirskRussia
  2. 2.Institute of Biological Problems of the NorthFar-Eastern Division of the Russian Academy of SciencesMagadanRussia
  3. 3.Institute of Chemical Biology and Fundamental MedicineSiberian Division of the Russian Academy of SciencesNovosibirskRussia

Personalised recommendations