Structure of O-antigen and functional characterization of O-antigen gene cluster of Salmonella enterica O47 containing ribitol phosphate and 2-acetimidoylamino-2,6-dideoxy-L-galactose
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An O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Salmonella enterica O47 and studied by sugar analysis along with one- and two-dimensional 1H- and 13C-NMR spectroscopy. The following structure of the linear ribitol phosphate-containing repeating unit of the O-polysaccharide was established: \( \to 2) - D - Ribitol - 5 - P - (O \to 6) - \alpha - D - Galp - (1 \to 3) - \alpha - L - FucpNAm - (1 \to 3) - \beta - D - GlcpNAc - (1 \to , \) where FucNAm stands for 2-acetimidoylamino-2,6-dideoxy-L-galactose. About 10% of Gal is O-acetylated at position 4 and another minor O-acetyl group is present at an undetermined position. Functions of the S. enterica O47 antigen biosynthetic genes were tentatively assigned by comparison with gene databases and found to be in agreement with the O-polysaccharide structure. A comparison of the O-antigen gene clusters of S. enterica O47 and E. coli O145 suggested their close evolutionary relationship.
Key wordsSalmonella enterica lipopolysaccharide bacterial polysaccharide structure acetimidoyl group ribitol phosphate O-antigen gene cluster
heteronuclear single-quantum coherence
rotating-frame nuclear Overhauser effect spectroscopy
total correlation spectroscopy