Biochemistry (Moscow)

, Volume 74, Issue 4, pp 399–405

Design of a novel interleukin-13 antagonist from analysis of informational structure

  • A. N. Nekrasov
  • L. E. Petrovskaya
  • V. A. Toporova
  • E. A. Kryukova
  • A. V. Rodina
  • E. Yu. Moskaleva
  • M. P. Kirpichnikov
Article

Abstract

Interleukin-13 (IL-13) is one of the cytokines involved in the development of Th2-type immune response. It plays an important role in the pathogenesis of asthma and other allergic diseases. Two deletion forms of IL-13 were constructed on a basis of informational structure analysis and expressed in E. coli cells. They were found to differ in ability to stimulate proliferation of TF-1 cell line. Deletion variant 146 (DV146) completely lacks such activity, whereas DV148 provides about 50% of the proliferation stimulation. The simultaneous addition of DV146 with full-length IL-13 suppresses proliferation depending on the concentration of the deletion form. Thus, the designed protein acts as an antagonist of IL-13.

Key words

ANIS method informational structure deletion variant IL-13 antagonist TF-1 proliferation 

Abbreviations

ANIS

analysis of informational structure

DV

deletion variant

ELIS

elements of informational structure

IDIC

increased degree of information coordination

IDIC-sites

sites with a locally increased degree of information coordination between residues

IL-13

interleukin-13

IPTG

isopropyl-β-D-thiogalactoside

IS

informational structure

IU

informational units

SOE

splice overlap extension

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • A. N. Nekrasov
    • 1
  • L. E. Petrovskaya
    • 1
  • V. A. Toporova
    • 1
  • E. A. Kryukova
    • 1
  • A. V. Rodina
    • 2
  • E. Yu. Moskaleva
    • 2
  • M. P. Kirpichnikov
    • 1
  1. 1.Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Department of Health CareMoscow Research Institute of Medical EcologyMoscowRussia

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