Advertisement

Biochemistry (Moscow)

, Volume 74, Issue 4, pp 362–370 | Cite as

T-cadherin activates Rac1 and Cdc42 and changes endothelial permeability

  • E. V. Semina
  • K. A. RubinaEmail author
  • P. N. Rutkevich
  • T. A. Voyno-Yasenetskaya
  • Y. V. Parfyonova
  • V. A. Tkachuk
Accelerated Publication

Abstract

In the present study, expression of T-cadherin was shown to induce intracellular signaling in NIH3T3 fibroblasts: it activated Rac1 and Cdc42 (p < 0.01) but not RhoA. T-Cadherin overexpression in human umbilical vein endothelial cells (HUVEC) using adenoviral constructs induced disassembly of microtubules and polymerization of actin stress fibers, whereas down-regulation of endogenous T-cadherin expression in HUVEC using lentiviral constructs resulted in micro-tubule polymerization and a decrease in the number of actin stress fibers. Moreover, suppression of the T-cadherin expression significantly decreased the endothelial monolayer permeability as compared to the control (p < 0.001).

Key words

T-cadherin Rho GTPases endothelial cell permeability microtubules actin 

Abbreviations

α-GAPDH

glyceraldehyde phosphate dehydrogenase

GFP

green fluorescent protein

HUVEC

human umbilical vein endothelial cells

shRNA

short hairpin RNA

siRNA

small interfering RNA

SRE

serum response element

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Supplementary material

10541_2009_4002_MOESM1_ESM.pdf (489 kb)
Supplementary material, approximately 340 KB.

References

  1. 1.
    Foty, R. A., and Steinberg, M. S. (2004) Intern. J. Dev. Biol., 48, 397–409.CrossRefGoogle Scholar
  2. 2.
    George, S. J., and Beeching, C. A. (2006) Atherosclerosis, 188, 1–11.PubMedCrossRefGoogle Scholar
  3. 3.
    Vestal, D. J., and Ranscht, B. (1992) J. Cell Biol., 119, 451–461.PubMedCrossRefGoogle Scholar
  4. 4.
    Philippova, M. P., Bochkov, V. N., Stambolsky, D. V., Tkachuk, V. A., and Resink, T. (1998) FEBS Lett., 129, 201–210.Google Scholar
  5. 5.
    Rubina, K. A., and Tkachuk, V. A. (2004) Russ. J. Physiol., 90, 968–986.Google Scholar
  6. 6.
    Rubina, K., Talovskaya, E., Cherenkov, V., Ivanov, D., Stambolsky, D., Storozhevykh, T., Pinelis, V., Shevelev, A., Parfyonova, Y., Resink, T., Erne, P., and Tkachuk, V. (2005) Mol. Cell Biochem., 273, 33–41.PubMedCrossRefGoogle Scholar
  7. 7.
    Ivanov, D., Philippova, M., Allenspach, R., Erne, P., and Resink, T. (2004) Cardiovasc. Res., 64, 132–143.PubMedCrossRefGoogle Scholar
  8. 8.
    Fredette, B. J., and Ranscht, B. (1994) J. Neurosci., 14, 7331–7346.PubMedGoogle Scholar
  9. 9.
    Fredette, B. J., Miller, J., and Ranscht, B. (1996) Development, 122, 3163–3171.PubMedGoogle Scholar
  10. 10.
    Rubina, K., Kalinina, N., Potekhina, A., Efimenko, A., Semina, E., Poliakov, A., Wilkinson, D. G., Parfyonova, Y., and Tkachuk, V. (2007) Angiogenesis, 10, 183–195.PubMedCrossRefGoogle Scholar
  11. 11.
    Wyder, L., Vitaliti, A., Schneider, H., Hebbard, L. W., Moritz, D. R., Wittmer, M., Ajmo, M., and Klemenz, R. (2000) Cancer Res., 60, 4682–4688.PubMedGoogle Scholar
  12. 12.
    Philippova, M., Ivanov, D., Allenspach, I., Takuwa, Y., Erne, P., and Resink, T. (2005) FASEB J., 19, 588–590.PubMedGoogle Scholar
  13. 13.
    Ivanov, D., Philippova, M., Tkachuk, V., Erne P., and Resink, T. (2004) Exp. Cell Res., 293, 207–218.PubMedCrossRefGoogle Scholar
  14. 14.
    Kudrjashova, E., Bashtrikov, P., Bochkov, V., Parfyonova, Y., Tkachuk, V., Antropova, J., Iljinskaya, O., Tararak, E., Erne, P., Ivanov, D., Philippova, M., and Resink, T. J. (2002) Histochem. Cell. Biol., 118, 281–290.PubMedGoogle Scholar
  15. 15.
    Philippova, M., Ivanov, D., Tkachuk, V., Erne, P., and Resink, T. J. (2003) Histochem. Cell Biol., 120, 353–360.PubMedCrossRefGoogle Scholar
  16. 16.
    Hordijk, P., Anthony, E., Mul, F., Rientsma, R., Oomen, L., and Roos, D. (1999) J. Cell Sci., 112, 1915–1923.PubMedGoogle Scholar
  17. 17.
    Hill, C. S., Wynne, J., and Treisman, R. (1995) Cell, 81, 1159–1170.PubMedCrossRefGoogle Scholar
  18. 18.
    Liu, G., and Voyno-Yasenetskaya, T. (2005) J. Biol. Chem., 280, 39042–39049.PubMedCrossRefGoogle Scholar
  19. 19.
    Ponimashkin, E. G., Profirovich, J., Vaskunaite, R., Ritcher, D. W., and Voyno-Yasenetskaya, T. (2002) J. Biol. Chem., 277, 20812–20819.CrossRefGoogle Scholar
  20. 20.
    Braga, V. M., del Maschio, A., Machesky, L., and Dejana, E. (1999) Mol. Biol. Cell, 10, 9–22.PubMedGoogle Scholar
  21. 21.
    Essler, M., Amano, M., Kruse, H.-J., Kaibuchi, K., Weber, P., and Aepfelbacher, M. (1998) J. Biol. Chem., 273, 21867–21874.PubMedCrossRefGoogle Scholar
  22. 22.
    Bishop, A. L., and Hall, A. (2000) Biochem. J., 348, 241–255.PubMedCrossRefGoogle Scholar
  23. 23.
    Wojciak-Stothard, B., and Ridley, A. J. (2003) Vasc. Pharmacol., 39, 187–199.CrossRefGoogle Scholar
  24. 24.
    Vouret-Cravari, V., Bourcier, C., Boutler, E., and van Obberghen-Schilling, E. (2002) J. Cell Sci., 115, 2475–2484.Google Scholar
  25. 25.
    Batard, P., Jordan, M., and Wurm, F. (2007) Gene, 270, 61–68.CrossRefGoogle Scholar
  26. 26.
    Gorovoy, M., Niu, J., Bernard, O., Profirovich, J., Minshall, J., Neamu, R., and Voyno-Yasenetskaya, T. (2005) J. Biol. Chem., 280, 26533–26542.PubMedCrossRefGoogle Scholar
  27. 27.
    Birukova, A., Smurova, K., Birukov, K., Usatyuk, P., Liu, F., Kaibuchi, K., Ricks-Cord, A., Natarajan, V., Alieva, I., Garcia, J. G. N., and Verin, A. D. (2004) J. Cell. Physiol., 201, 55–70.PubMedCrossRefGoogle Scholar
  28. 28.
    Bogatcheva, N., Adyshev, D., Mambetsariev, B., Moldobaeva, N., and Verin, A. (2007) Am. J. Physiol. Lung Cell. Mol. Physiol., 292, 487–499.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • E. V. Semina
    • 1
  • K. A. Rubina
    • 2
    Email author
  • P. N. Rutkevich
    • 1
  • T. A. Voyno-Yasenetskaya
    • 3
  • Y. V. Parfyonova
    • 1
  • V. A. Tkachuk
    • 2
  1. 1.Institute of Experimental CardiologyCardiology Research CenterMoscowRussia
  2. 2.Faculty of Fundamental MedicineLomonosov Moscow State UniversityMoscowRussia
  3. 3.Department of PharmacologyIllinois UniversityChicagoUSA

Personalised recommendations