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Biochemistry (Moscow)

, Volume 74, Issue 3, pp 336–341 | Cite as

Polyclonal antibodies to LIM proteins CRP2 and CRIP2 reveal their subcellular localizations in olfactory precursor cells

  • Xue Gao
  • Jing-Yu Sun
  • Zeng-Yu Cao
  • Ying Lin
  • Ding-Jun Zha
  • Feng Wang
  • Tao Xue
  • Li Qiao
  • Lian-Jun Lu
  • Jian-Hua QiuEmail author
Article
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Abstract

In this study, we describe the presence of CRP2 (cysteine- and glycine-rich protein 2) and CRIP2 (cysteine-rich intestinal protein 2), which are members of group 2 LIM proteins, in rat olfactory precursor cells by reverse transcription polymerase chain reaction. We have developed polyclonal antibodies against CRP2 and CRIP2 individually. Specificity of the antibodies was demonstrated by Western blot analysis, using CRP2 and CRIP2 transfected cells. No cross-reactivity was observed between the antibodies. Furthermore, we used the antibodies to determine the expression and localization of CRP2 and CRIP2 in olfactory precursor cells by Western blot analysis and immunofluorescence staining. Our results demonstrated that in undifferentiated olfactory precursor cells CRP2 was distributed both in the nucleus and the cytoplasm, whereas CRIP2 was predominantly localized in the cytoplasm. While the olfactory precursor cells differentiated into end cells, only the expression of CRIP2 would be detected. The function of these LIM proteins in olfactory precursor cells warrants further study.

Key words

CRP2 CRIP2 LIM protein polyclonal antibody olfactory precursor cells 

Abbreviations

CRIPs

cysteine-rich intestinal proteins

CRPs

cysteine- and glycine-rich proteins

ELISA

enzyme-linked immunosorbent assay

FCS

fetal calf serum

IPTG

isopropyl-β-D-thiogalactopyranoside

OB

olfactory bulb

PBS

phosphate-buffered saline

RT-PCR

reverse transcription polymerase chain reaction

SVZ

subventricular zone

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • Xue Gao
    • 1
  • Jing-Yu Sun
    • 1
  • Zeng-Yu Cao
    • 1
  • Ying Lin
    • 1
  • Ding-Jun Zha
    • 1
  • Feng Wang
    • 1
  • Tao Xue
    • 1
  • Li Qiao
    • 1
  • Lian-Jun Lu
    • 1
  • Jian-Hua Qiu
    • 1
    Email author
  1. 1.Department of Otorhinolaryngology, Head and Neck Surgery, Xijing HospitalFourth Military Medical UniversityXi’anP. R. China

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