Biochemistry (Moscow)

, Volume 74, Issue 3, pp 329–335 | Cite as

A lectin from Sesbania aculeata (Dhaincha) roots and its possible function

  • S. Biswas
  • A. Saroha
  • H. R. DasEmail author


A lectin was isolated from the roots of Sesbania aculeata. This is a glucose specific lectin having 39 kDa subunit molecular weight. The expression of this lectin was found to be developmentally regulated and observed to be the highest in the second week. The lectin was purified by affinity chromatography using Sephadex G-50 and found to have 28% homology with Arabidopsis thaliana lectin-like protein (accession No. CAA62665). The lectin binds with lipopolysaccharide isolated from different rhizobial strains indicating the plants interaction with multiple rhizobial species.

Key words

Sesbania root lectin lipopolysaccharides 



autoclaved distilled water


two-dimensional polyacrylamide gel electrophoresis


enzyme linked lectin binding assay






peptide mass fingerprinting


rabbit erythrocytes


Sesbania root agglutinin


Tris-buffer solution


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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  1. 1.Institute of Genomics and Integrative BiologyDelhi University CampusDelhiIndia
  2. 2.Faculty of Natural SciencesWest Bengal University of TechnologyKolkataIndia

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