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Biochemistry (Moscow)

, Volume 74, Issue 3, pp 329–335 | Cite as

A lectin from Sesbania aculeata (Dhaincha) roots and its possible function

  • S. Biswas
  • A. Saroha
  • H. R. DasEmail author
Article

Abstract

A lectin was isolated from the roots of Sesbania aculeata. This is a glucose specific lectin having 39 kDa subunit molecular weight. The expression of this lectin was found to be developmentally regulated and observed to be the highest in the second week. The lectin was purified by affinity chromatography using Sephadex G-50 and found to have 28% homology with Arabidopsis thaliana lectin-like protein (accession No. CAA62665). The lectin binds with lipopolysaccharide isolated from different rhizobial strains indicating the plants interaction with multiple rhizobial species.

Key words

Sesbania root lectin lipopolysaccharides 

Abbreviations

ADW

autoclaved distilled water

2D-PAGE

two-dimensional polyacrylamide gel electrophoresis

ELLBA

enzyme linked lectin binding assay

HA

hemagglutination

LPS

lipopolysaccharide

PMF

peptide mass fingerprinting

RRBC

rabbit erythrocytes

SRA

Sesbania root agglutinin

TBS

Tris-buffer solution

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References

  1. 1.
    Goldstein, I. J., Hughes, R. C., Monsigny, M., Osawa, and Sharon, T. N. (1980) Nature, 285, 66.CrossRefGoogle Scholar
  2. 2.
    Van Damme, E. J. M., Peumans, W. J., Pusztai, A., and Bardocz, S. (1998) Handbook of Plant Lectins: Properties and Biomedical Applications, John Wiley, England.Google Scholar
  3. 3.
    Rouge, P., and Pere, D. (1982) in Lectins. Biology, Biochemistry, Clinical Biochemistry (Bog Hensen, T. C., ed.) Vol. II, Walter de Gruyter, Berlin, pp. 137–150.Google Scholar
  4. 4.
    Etzler, M. E. (1986) in The Lectins: Properties, Functions and Applications in Biology and Medicine (Liener, I. E., Sharon, N., and Goldstein, I. J., eds.) Academic Press, New York, pp. 371–435.Google Scholar
  5. 5.
    Jayaraman, V., and Das, H. R. (1998) Biochim. Biophys. Acta, 138, 17–11.Google Scholar
  6. 6.
    Bhattacharya, I., Biswas, S., Das, R. H., and Das, H. R. (2004) Ind. J. Biochem. Biophys., 41, 89–95.Google Scholar
  7. 7.
    Kijne, J. W., Bauchrowitz, M. A., and Diaz, C. L. (1997) Plant Physiol., 115, 869–873.PubMedGoogle Scholar
  8. 8.
    Brewin, N. J. (1998) in The Rhizobiaceae (Spaink, H. P., Kondorosi, A., and Hooykaas, P. J. J., eds.) Kluwer Academic Publishers, Dordrecht, pp. 417–429.Google Scholar
  9. 9.
    Gage, D. J., and Margolin, W. (2000) Curr. Opin. Microbiol., 3, 613–617.PubMedCrossRefGoogle Scholar
  10. 10.
    Sharma, R. S., Mohmmed, A., Mishra, V., and Babu, C. R. (2005) Res. Microbiol., 156, 57–67.PubMedCrossRefGoogle Scholar
  11. 11.
    Ali, Z., Zahid, M. S., Hussain, A., Khan, S., Hassan, M. Z., and Bashir, M. (2004) Agridigest, 25, 39–44.Google Scholar
  12. 12.
    Mohammad, A., Jamshid, H. R., Khan, E. A., and Mohammad, R. (2005) J. Res. Sci. (Pakistan), 16, 39–43.Google Scholar
  13. 13.
    Sharma, A. R., and Ghosh, A. (2000) Nutr. Cycl. Agroecosyst., 57, 141–153.CrossRefGoogle Scholar
  14. 14.
    Gaur, Y. D., and Sen, A. N. (1979) New Phytol., 83, 745–754.CrossRefGoogle Scholar
  15. 15.
    Grant, G., More, L. J., Mc Kenzie, N. H., Stewart, J. C., and Pusztai, A. (1983) Br. J. Nutr., 50, 207–214.PubMedCrossRefGoogle Scholar
  16. 16.
    Pugalenthi, M., Vadivel, V., Gurumoorthi, P., and Janardhanan, K. (2004) Trop. Subtrop. Agroecosyst., 4, 107–123.Google Scholar
  17. 17.
    Lowry, O. H., Rosenbrough, N. J., Lewis, F. A., and Randall, R. J. (1951) J. Biol. Chem., 1936, 265–275.Google Scholar
  18. 18.
    Kalsi, G., Das, H. R., Babu, C. R., and Das, R. H. (1992) Biochim. Biophys. Acta, 117, 114–119.Google Scholar
  19. 19.
    Davis, B. J. (1964) Ann. N. Y. Acad. Sci., 121, 404–427.PubMedCrossRefGoogle Scholar
  20. 20.
    Laemmli, U. K. (1970) Nature, 227, 680–685.PubMedCrossRefGoogle Scholar
  21. 21.
    Hernandez, P., Debray, H., Jaekel, H., Garfias, Y., Jimenez, Md. Mdel. C., Martinez-Cairo, S., and Zenteno, E. (2001) Glycoconj. J., 18, 321–329.PubMedCrossRefGoogle Scholar
  22. 22.
    Flensburg, J., Haid, D., Blomberg, J., Bielawski, J., and Ivansson, D. (2004) J. Biochem. Biophys. Meth., 60, 319–334.PubMedCrossRefGoogle Scholar
  23. 23.
    Westphal, O., and Jann, K. (1965) Meth. Carbohydr. Chem., 5, 83–91.Google Scholar
  24. 24.
    Borrebaeck, C. A. K. (1984) Planta, 161, 223–228.CrossRefGoogle Scholar
  25. 25.
    Spilatro, S. R., Cochran, G. R., Walker, R. E., Cablish, K., and Bittner, C. C. (1996) Plant Physiol., 110, 825–834.PubMedCrossRefGoogle Scholar
  26. 26.
    Diaz, C. L., Melchers, L. S., Hooykaas, P. J. J., Lugtenberg, E. J. J., and Kijne, J. W. (1989) Nature, 338, 579–581.CrossRefGoogle Scholar
  27. 27.
    Sharon, N., and Lis, H. (2004) Glycobiology, 14, 53R–62R.PubMedCrossRefGoogle Scholar
  28. 28.
    Pathak, M., Singh, B., Sharma, A., Agrawal, P., Pasha, S. B., Das, H. R., and Das, R. H. (2006) Plant Mol. Biol., 62, 529–545.PubMedCrossRefGoogle Scholar
  29. 29.
    Link, A. J., Eng, J., Schieltz, D. M., Carmack, E., Mize, G. J., Morris, D. R., Garvik, B. M., III, and Yates, J. R. (1999) Nat. Biotechnol., 17, 676–682.PubMedCrossRefGoogle Scholar
  30. 30.
    Rittidach, W., Paijit, N., and Utarabhand, P. (2007) Biochim. Biophys. Acta, 1770, 106–114.PubMedGoogle Scholar
  31. 31.
    Goldstein, I. J., and Poretz, R. D. (1986) in The Lectins: Properties, Functions and Applications in Biology and Medicines (Liener, I. E., Sharon, N., Goldstein, I. J., eds.) Academic Press, New York, pp. 33–247.Google Scholar
  32. 32.
    Singh, R., and Das, H. R. (1994) Glycoconj. J., 11, 282–285.PubMedCrossRefGoogle Scholar
  33. 33.
    Van Rhijn, P., and Vanderleyden, J. (1995) Microbiol. Rev., 59, 124–142.PubMedGoogle Scholar
  34. 34.
    Biswas, S., Das, R. H., Sharma, G. L., and Das, H. R. (2008) Curr. Microbiol., 56, 48–54.PubMedCrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  1. 1.Institute of Genomics and Integrative BiologyDelhi University CampusDelhiIndia
  2. 2.Faculty of Natural SciencesWest Bengal University of TechnologyKolkataIndia

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