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Biochemistry (Moscow)

, Volume 74, Issue 3, pp 301–307 | Cite as

Dihydroquercetin (taxifolin) and other flavonoids as inhibitors of free radical formation at key stages of apoptosis

  • Yu. A. VladimirovEmail author
  • E. V. Proskurnina
  • E. M. Demin
  • N. S. Matveeva
  • O. B. Lubitskiy
  • A. A. Novikov
  • D. Yu. Izmailov
  • A. N. Osipov
  • V. P. Tikhonov
  • V. E. Kagan
Article

Abstract

Formation of free radicals in mitochondria plays a key role in the development of apoptosis, which includes formation of superoxide by the respiratory chain, formation of radicals by cytochrome c-cardiolipin complex in the presence of hydrogen peroxide or lipids, and chain lipid peroxidation resulting in cytochrome c release from mitochondria and initiation of the apoptotic cascade. In this work the effect of taxifolin (dihydroquercetin) and some other antioxidants on these three radical-producing reactions was studied. Peroxidase activity of the complex of cytochrome c with dioleyl cardiolipin estimated by chemiluminescence with luminol decreased by 50% with quercetin, taxifolin, rutin, Trolox, and ionol at concentrations 0.7, 0.7, 0.8, 3, and 10 μM, respectively. The lipid radical production detected by coumarin C-525-activated chemiluminescence decreased under the action of rutin and taxifolin in a dose-dependent manner, so that a 50% inhibition of chemiluminescence was observed at the antioxidant concentrations of 3.7 and 10 μM, respectively. Thus, these two radical-producing reactions responsible for apoptosis onset are inhibited by antioxidants at rather low concentrations. Experiments performed on liver slices and mash showed that taxifolin, quercetin, naringenin, and Trolox have low inhibitory effect on the lucigenin-dependent chemiluminescence in the tissue only at concentrations higher than 100 μM.

Key words

dihydroquercetin antioxidants apoptosis free-radical reactions cytochrome c cardiolipin 

Abbreviations

BCL

bovine cardiolipin

CL

chemiluminescence

coumarin C-525

2,3,5,6-1H,4H-tetrahydro-9-(2′-benzoimidazolyl)quinolizino-(9,9a,1-gh)coumarin

DHQ

dihydroquercetin (taxifolin)

NO

nitric oxide

SOD

superoxide dismutase

TOCL

1,1′,2,2′-tetraoleyl cardiolipin (sodium salt)

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • Yu. A. Vladimirov
    • 1
    Email author
  • E. V. Proskurnina
    • 1
  • E. M. Demin
    • 1
  • N. S. Matveeva
    • 2
  • O. B. Lubitskiy
    • 2
  • A. A. Novikov
    • 2
  • D. Yu. Izmailov
    • 1
  • A. N. Osipov
    • 2
  • V. P. Tikhonov
    • 3
  • V. E. Kagan
    • 4
  1. 1.Faculty of Basic MedicineLomonosov Moscow State UniversityMoscowRussia
  2. 2.Russian State Medical UniversityMoscowRussia
  3. 3.Moscow Plant “Diod”MoscowRussia
  4. 4.University of PittsburghPittsburghUSA

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