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Biochemistry (Moscow)

, Volume 74, Issue 3, pp 288–292 | Cite as

Metabolic control analysis of L-cysteine producing strain TS1138 of Pseudomonas sp.

  • Lihua HuaiEmail author
  • Ning Chen
  • Wenbo Yang
  • Gang Bai
Article

Abstract

A kinetic model describing the biosynthesis of L-cysteine by Pseudomonas sp. TS1138 has been developed. The two enzymes catalyzing this pathway, L-cysteine synthetase (CS) and L-cysteine desulfhydrase (CD), follow Michaelis-Menten kinetics with noncompetitive inhibition of CS by L-cysteine. From measurements of intermediates and end products that were made during L-cysteine enzymatic synthesis, metabolic control analysis of the pathway was carried out using the kinetic model. The elasticity coefficients and the flux control coefficients were calculated, and the analysis revealed a shift in the flux control from CS to CD during the reaction. The findings further implicate potential targets and strategies for increasing L-cysteine production; for example, the strain TS1138 could be manipulated by site-directed mutagenesis to reduce CD activity.

Key words

Pseudomonas sp. TS1138 L-cysteine kinetic model metabolic control analysis enzymatic synthesis 

Abbreviations

CD

L-cysteine desulfhydrase

CS

L-cysteine synthetase

DL-ATC

DL-2-amino-Δ2-thiazoline-4-car-boxylic acid

MCA

metabolic control analysis

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  1. 1.College of BiotechnologyTianjin University of Science and TechnologyTianjinP. R. China
  2. 2.Tianjin University of CommerceTianjinP. R. China
  3. 3.College of Life ScienceNanKai UniversityTianjinP. R. China

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