Biochemistry (Moscow)

, Volume 74, Issue 3, pp 268–275 | Cite as

Role of extracellular calcium ions at early stages of human T-lymphocyte polyclonal activation in vitro

  • I. S. LitvinovEmail author
  • M. A. Fomichev
  • M. P. Kirpichnikov


In this work we comparatively analyzed interleukin-2 (IL-2) and interferon γ production (IFN-γ) and also CD69 and CD25 expression by activated T-cells depending on extracellular calcium concentration ([Ca2+]e), which was varied with EGTA. The expression of CD69 molecules on the surface of T-cells depended only on the presence of phorbol myristate acetate, occurred at [Ca2+]e higher than 0.2 mM, and did not require the presence of ionomycin. The increase in [Ca2+]e by itself cannot induce expression of CD25 and CD69 molecules by activated cells. The values of [Ca2+]e, at which maximal fractions of CD3+CD69+(IL-2)+, CD3+CD69+(IFN-γ)+, and CD3+CD25+ activated T-cells were reached, never coincided with mean values of [Ca2+]e for healthy donors and were different from each other. So, there is different [Ca2+]e dependence for initial stages of activated T-cells differentiation. The relation between T-cells activation parameters and their differentiation is discussed.

Key words

human peripheral blood ionomycin PMA cell activation EGTA IL-2 IFN-γ 



extracellular calcium concentration


intracellular calcium concentration


cell differentiation cluster


concanavalin A


fetal bovine serum


human peripheral blood


interferon γ




ionomycin-resistant cells


monoclonal antibodies


phosphate-buffered saline


haricot bean phytohemagglutinin


phorbol myristate acetate


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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • I. S. Litvinov
    • 1
    Email author
  • M. A. Fomichev
    • 2
  • M. P. Kirpichnikov
    • 1
  1. 1.Shemyakin and Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia
  2. 2.Moscow State Medical Stomatological UniversityMoscowRussia

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