Biochemistry (Moscow)

, Volume 74, Issue 2, pp 201–208 | Cite as

Hemoglobin as a potential source of natural regulatory oligopeptides

  • A. A. ZamyatninEmail author


Theoretical structure-function analysis of all possible hemoglobin molecule fragments was performed to determine sites that could be potential sources of regulatory oligopeptides. Known data on bovine hemoglobin primary structure and information of the EROP-Moscow database concerning structure and functions of natural oligopeptides were used along with a computer program complex. A total of 6750 natural non-hemoglobin oligopeptides with hemoglobin fragments of 2–14 amino acid residues were found. Structures of 20 of them were completely identical to hemoglobin fragments. Most of the revealed oligopeptides exhibit properties of neuropeptides, antimicrobial agents, and hormones. A number of them exhibit functions previously not known for hemoglobin fragments. The possibility of natural formation of regulatory oligopeptides from hemoglobin and other food protein molecules, generation of the exogenous oligopeptide pool, their participation in regulation processes as well as accordance of results obtained here with the oligopeptide continuum concepts are discussed.

Key words

hemoglobin fragment neuropeptide antimicrobial oligopeptide EROP-Moscow database continuum 





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  1. 1.
    Zamyatnin, A. A. (1984) Ann. Rev. Biophys. Bioeng., 13, 145–165.CrossRefGoogle Scholar
  2. 2.
    Zamyatnin, A. A. (1991) Prot. Seq. Data Anal., 4, 49–52.Google Scholar
  3. 3.
    Zamyatnin, A. A., Borchikov, A. S, Vladimirov, M. G., and Voronina, O. L. (2005) Neirokhimiya, 22, 12–27.Google Scholar
  4. 4.
    Zamyatnin, A. A. (2004) Endogenous Regulatory OligoPeptide Knowledgebase,
  5. 5.
    Brantl, V., Teschemacher, H., Henschen, A., and Lottspeich, F. (1979) Hoppe-Seyler’s Z. Physiol. Chem., 360, 1211–1216.PubMedGoogle Scholar
  6. 6.
    Henschen, A., Lottspeich, F., Brantl, V., and Teschemacher, H. (1979) Hoppe-Seyler’s Z. Physiol. Chem., 360, 1217–1224.PubMedGoogle Scholar
  7. 7.
    Brantl, V., and Teschemacher, H. (1983) Trends Pharmacol. Sci., 4, 193–193.Google Scholar
  8. 8.
    Brantl, V. (1984) Eur. J. Pharmacol., 106, 213–214.PubMedCrossRefGoogle Scholar
  9. 9.
    Koch, G., Wiedemann, K., and Teschemacher, H. (1985) Naunyn-Schmiedeberg’s Arch. Pharmacol., 331, 351–354.CrossRefGoogle Scholar
  10. 10.
    Teschemacher, H., Koch, G., and Brantl, V. (1997) Biopolymers. Peptide Sci., 43, 99–117.CrossRefGoogle Scholar
  11. 11.
    Meisel, H. (1997) Biopolymers. Peptide Sci., 43, 119–128.CrossRefGoogle Scholar
  12. 12.
    Brantl, V., Gramsch, Ch., Lottspeich, F., Henschen, A., Jaeger, K. H., and Herz, A. (1985) Eur. J. Pharmacol., 111, 293.PubMedCrossRefGoogle Scholar
  13. 13.
    Zadina, J. E., Kastin, A. J., Ge, L. J., and Brantl, V. (1990) Life Sci., 47, PL25–30.PubMedCrossRefGoogle Scholar
  14. 14.
    Brantl, V., Gramsch, Ch., Lottspeich, F., Mertz, R., Jaeger, K. H., and Herz, A. (1986) Eur. J. Pharmacol., 125, 309–310.PubMedCrossRefGoogle Scholar
  15. 15.
    Zhao, Q., Garreau, I., Sannier, F., and Piot, J. M. (1997) Biopolymers. Peptide Sci., 43, 75–98.CrossRefGoogle Scholar
  16. 16.
    Nyberg, F., Sanderson, K., and Glamsta, E.-L. (1997) Biopolymers. Peptide Sci., 43, 147–156.CrossRefGoogle Scholar
  17. 17.
    Ivanov, V. T., Karelin, A. A., Philippova, M. M., Nazimov, I. V., and Pletnev, V. Z. (1997) Biopolymers. Peptide Sci., 43, 171–188.CrossRefGoogle Scholar
  18. 18.
    Ueta, E., Tanida, T., and Osaki, T. (1997) Biopolymers. Peptide Sci., 43, 240–249.Google Scholar
  19. 19.
    Froidevaux, R., Krier, F., Nedjar-Arroume, N., Vercaigne-Marko, D., Kosciarz, E., Ruckebusch, C., Dhulster, P., and Guillochon, D. (2001) FEBS Lett., 491, 159–163.PubMedCrossRefGoogle Scholar
  20. 20.
    Fogaca, A. C., da Silva, P. I., Jr., Miranda, M. T., Bianchi, A. G., Miranda, A., Ribolla, P. E., and Daffre, S. (1999) J. Biol. Chem., 274, 25330–25334.PubMedCrossRefGoogle Scholar
  21. 21.
    Ivanov, V. T., Karelin, A. A., Mikhaleva, I. I., Vas’kovskii, B. V., Svryaev, V. I., and Nazimov, I. V. (1992) Bioorg. Khim., 18, 1271–1310.Google Scholar
  22. 22.
    Takagi, H., Shiomi, H., Ueda, H., and Amano, H. (1979) Nature, 282, 410–412.PubMedCrossRefGoogle Scholar
  23. 23.
    Piot, J.-M., Zhao, Q., Guillochon, D., Ricart, G., and Thomas, D. (1992) FEBS Lett., 299, 75–79.PubMedCrossRefGoogle Scholar
  24. 24.
    Glamsta, E.-L., Marklund, A., Hellman, U., Wernstedt, C., Terenius, L., and Nyberg, F. (1991) Regul. Pept., 34, 169–179.PubMedCrossRefGoogle Scholar
  25. 25.
    Barkhudaryan, N., Oberthuer, W., Lottspeich, F., and Galoyan, A. (1992) Neurochem. Res., 17, 1217–1221.PubMedCrossRefGoogle Scholar
  26. 26.
    Dubois, V., Nedjar-Arroume, N., and Guillochon, D. (2005) Prepar. Biochem. Biotechnol., 35, 85–102.CrossRefGoogle Scholar
  27. 27.
    Chang, R. C. C., Huang, W.-Y., Redding, T. W., Arimura, A., Coy, D. H., and Schally, A. V. (1980) Biochim. Biophys. Acta, 625, 266–273.PubMedGoogle Scholar
  28. 28.
    Zamyatnin, A. A., Borchikov, A. S., Vladimirov, M. G., and Voronina, O. L. (2006) Nucleic Acids Res., 34, D261–D266.PubMedCrossRefGoogle Scholar
  29. 29.
    Schroeder, W. A., Shelton, J. R., Shelton, J. B., Robberson, B., and Babin, D. R. (1967) Arch. Biochem. Biophys., 120, 1–14.PubMedCrossRefGoogle Scholar
  30. 30.
    Schroeder, W. A., Shelton, J. R., Shelton, J. B., Robberson, B., and Babin, D. R. (1967) Arch. Biochem. Biophys., 120, 124–135.PubMedCrossRefGoogle Scholar
  31. 31.
    Ianzer, D., Konno, K., Xavier, C. H., Stocklin, R., Santos, R. A., de Camargo, A. C., and Pimenta, D. C. (2006) Peptides, 27, 2957–2966.PubMedCrossRefGoogle Scholar
  32. 32.
    Ziganshin, R. Kh., Sviryaev, V. I., Vas’kovskii, B. V., Mikhaleva, I. I., Ivanov, V. T., Kokoz, Yu. M., Alexeev, A. E., Korystova, A. F., Sukhova, E. G., Ulyanova, E. G., and Usenko, A. B. (1994) Bioorg. Khim., 20, 899–918.PubMedGoogle Scholar
  33. 33.
    Yavin, E. J., Preciado-Patt, L., Rosen, O., Yaron, M., Suessmuth, R. D., Levartowsky, D., Jung, G., Lider, O., and Fridkin, M. (2000) FEBS Lett., 472, 259–262.PubMedCrossRefGoogle Scholar
  34. 34.
    Li, C. H., and Chung, D. (1976) Proc. Natl. Acad. Sci. USA, 73, 1145–1148.PubMedCrossRefGoogle Scholar
  35. 35.
    Hughes, J., Smyth, T. W., Kosterlitz, H. W., Fothergill, L. A., Morgan, B. A., and Morris, H. R. (1975) Nature, 258, 577–579.PubMedCrossRefGoogle Scholar
  36. 36.
    Oren, Z., and Shai, Y. (1996) Eur. J. Biochem., 237, 303–310.PubMedCrossRefGoogle Scholar
  37. 37.
    Shai, Y., Fox, J., Caratsch, C., Shuh, Y.-L., Edwards, C., and Lazarovici, P. (1988) FEBS Lett., 242, 161–166.PubMedCrossRefGoogle Scholar
  38. 38.
    Harris, J. I., and Lerner, A. B. (1957) Nature, 179, 1346–1347.PubMedCrossRefGoogle Scholar
  39. 39.
    Lee, T. H., Lerner, A. B., and Buettner-Janusch, V. (1961) J. Biol. Chem., 236, 2970–2974.PubMedGoogle Scholar
  40. 40.
    Graf, L., Bajusz, S., Patty, A., Barat, E., and Cseh, G. (1971) Acta Biochim. Biophys. Acad. Sci. Hung., 6, 415–418.PubMedGoogle Scholar
  41. 41.
    Takagi, H., Shiomi, H., Fukui, K., Hayashi, K., Kiso, Y., and Kitagawa, K. (1982) Life Sci., 31, 1733–1736.PubMedCrossRefGoogle Scholar
  42. 42.
    Orts, R. J., Liao, T.-H., Sartin, J. L., and Bruot, B. (1978) Physiologist, 21, 87–87.Google Scholar
  43. 43.
    Takagi, H., Shiomi, H., Ueda, H., and Amano, H. (1979) Nature, 282, 410–412.PubMedCrossRefGoogle Scholar
  44. 44.
    Ashmarin, I. P., and Obukhova, M. F. (1986) Biokhimiya, 51, 531–544.Google Scholar
  45. 45.
    Zamyatnin, A. A., and Voronina, O. L. (1998) Uspekhi Biol. Khim., 38, 165–197.Google Scholar
  46. 46.
    Zamyatnin, A. A., and Borchikov, A. S. (2007) Neurochem. J., 1, 188–195.CrossRefGoogle Scholar

Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  1. 1.Bach Institute of BiochemistryRussian Academy of SciencesMoscowRussia
  2. 2.Departamento de InformaticaUniversidad Tecnica Federico Santa MariaValparaisoChile

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