Biochemistry (Moscow)

, Volume 74, Issue 2, pp 162–164 | Cite as

Adsorption chromatography of proteins

  • V. I. Surovtsev
  • V. M. BorzenkovEmail author
  • K. V. Detushev


A method for adsorption chromatography of proteins is proposed. A protein solution is passed through a cellulose column at a pH value corresponding to an isoelectric point of the protein. Depending on the charge of unwanted proteins, they either remain at the origin (if charges of protein and ion-exchanger are opposite) or are released from the column (if charges of protein and ion-exchanger coincide). Elution volume of the purified protein is higher than for the second group of unwanted proteins because movement of the uncharged protein of interest includes its adsorption on cellulose followed by subsequent desorption caused by the elution buffer. Problems of optimization of buffers and adsorbents are discussed. Applicability of the method of adsorption chromatography is illustrated using purification of horseradish peroxidase as an example.

Key words

adsorption chromatography adsorbent-ion exchanger cellulose buffer peroxidase amine 



3-(N-morpholino)propanesulfonic acid


3-(N-morpholino)-2-hydroxypropanesulfonic acid




N-tris(hydroxymethyl)methyl-2-aminosulfonic acid


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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • V. I. Surovtsev
    • 1
  • V. M. Borzenkov
    • 1
    Email author
  • K. V. Detushev
    • 1
  1. 1.State Research Center of Applied Microbiology and BiotechnologyMinistry of Health and Social Development of the Russian FederationObolensk, Moscow RegionRussia

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