Biochemistry (Moscow)

, Volume 74, Issue 1, pp 92–96 | Cite as

Replication protein A modulates the activity of human telomerase in vitro

  • M. P. Rubtsova
  • D. A. Skvortsov
  • I. O. Petruseva
  • O. I. Lavrik
  • P. V. Spirin
  • V. S. Prasolov
  • F. L. Kisseljov
  • O. A. DontsovaEmail author


Our aim was to investigate how replication protein A (RPA) in a wide range of concentration can regulate the activity of human telomerase. We used an in vitro system based on human cell extracts with or without RPA. It has been shown that removal of RPA leads to loss of telomerase activity and addition of RPA restores telomerase activity and at the same time promotes telomerase processivity. However, high excess of RPA inhibited telomerase processivity and caused the synthesis of relatively short DNA fragments (about 50–100 nucleotides). We assume that, together with other telomere-binding proteins, RPA may take part in activation of telomere overhang elongation by telomerase at a certain stage of a cell cycle as well as in regulation of telomere length.

Key words

telomerase RPA telomeres regulation 





replication protein A


single-strand binding protein


single-strand DNA


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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • M. P. Rubtsova
    • 1
  • D. A. Skvortsov
    • 1
  • I. O. Petruseva
    • 2
  • O. I. Lavrik
    • 2
  • P. V. Spirin
    • 3
  • V. S. Prasolov
    • 3
  • F. L. Kisseljov
    • 4
  • O. A. Dontsova
    • 1
    Email author
  1. 1.Chemical FacultyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Institute of Chemical Biology and Fundamental MedicineSiberian Division of the Russian Academy of SciencesNovosibirskRussia
  3. 3.Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia
  4. 4.Blokhin Cancer Research CenterRussian Academy of Medical SciencesMoscowRussia

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