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Biochemistry (Moscow)

, Volume 74, Issue 1, pp 61–67 | Cite as

Role of OsHAL3 protein, a putative 4′-phosphopantothenoylcysteine decarboxylase in rice

  • Ning Zhang
  • Xuechen Wang
  • Jia ChenEmail author
Article

Abstract

In this study, we cloned the OsHAL3 gene from rice Oryza sativa. Alignment analysis revealed that OsHAL3 has a high sequence identity to Dfp protein in Escherichia coli and AtHAL3a protein in Arabidopsis thaliana, which have 4′-phosphopantothenoylcysteine decarboxylase (PPC-DC) activity. OsHAL3 can complement mutation in the E. coli dfp gene encoding PPC-DC, so that the mutant strains with OsHAL3 can grow on rich media at 42°C and on VB minimal media at 30°C. Complementation tests with point mutations of OsHAL3 suggested that the conserved Cys176 residue of OsHAL3 is a key active-site residue. The mutant OsHAL3 G180A has a partly reduced activity. Related mRNA-level analysis showed that the OsHAL3 gene is induced by calcium pantothenate in rice.

Key words

OsHAL3 4′-phosphopantothenoylcysteine decarboxylase coenzyme A rice 

Abbreviations

CoA

coenzyme A

MS

Murashige and Skoog basal salt mixture

PP

4′-phosphopantetheine

PPC

(R)-4′-phospho-N-pantothenoylcysteine

PPC-DC

4′-phosphopan-tothenoylcysteine decarboxylase

TY

tryptone-yeast medium

VB

Vogel-Bonner medium

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Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  1. 1.State Key Laboratory of Plant Physiology and Biochemistry, College of Biological SciencesChina Agricultural UniversityBeijingChina
  2. 2.Laboratory of Development Biology, Biological Science and Technology CollegeShenyang Agricultural UniversityShengyangChina

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