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Biochemistry (Moscow)

, Volume 73, Issue 11, pp 1224–1227 | Cite as

Efflux of potassium ions from cells and spheroplasts of Saccharomyces cerevisiae yeast treated with silver and copper ions

  • V. M. Vagabov
  • A. Yu. Ivanov
  • T. V. KulakovskayaEmail author
  • E. V. Kulakovskaya
  • V. V. Petrov
  • I. S. Kulaev
Article

Abstract

Silver ions induce the efflux of potassium from cells of the yeast Saccharomyces cerevisiae but have no such effect on spheroplasts. Copper ions and the natural fungicide 2-O-3-hydroxyhexanoyl-β-D-glucopyranosyl-(1→4)-(6-O-acetyl-β-D-glucopyranosyl-(1→16)-2,15,16-trihydroxyhexadecanoic acid) induce the efflux of potassium ions from both cells and spheroplasts of S. cerevisiae. Silver and copper ions inhibit the activity of the plasma membrane H+-ATPase during the treatment of both cells and spheroplasts. It is supposed that the inability of silver ions to stimulate potassium efflux from spheroplasts results from damage to some components of K+ transport systems during preparation of spheroplasts.

Key words

potassium silver copper cell wall plasma membrane H+-ATPase ion leakage yeast Saccharomyces cerevisiae 

Abbreviations

CL

cellobiose lipid 2-O-3-hydroxyhexanoyl-β-D-glucopyranosyl-(1→4)-(6-O-acetyl-β-D-glucopyranosyl-(1→16)-2,15,16-trihydroxyhexadecanoic acid)

EKP

electrokinetic potential

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Copyright information

© MAIK Nauka 2008

Authors and Affiliations

  • V. M. Vagabov
    • 1
  • A. Yu. Ivanov
    • 2
  • T. V. Kulakovskaya
    • 1
    Email author
  • E. V. Kulakovskaya
    • 1
  • V. V. Petrov
    • 1
  • I. S. Kulaev
    • 1
  1. 1.Skryabin Institute of Biochemistry and Physiology of MicroorganismsRussian Academy of SciencesPushchino, Moscow RegionRussia
  2. 2.Institute of Cell BiophysicsRussian Academy of SciencesPushchino, Moscow RegionRussia

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