Biochemistry (Moscow)

, Volume 73, Issue 11, pp 1192–1199 | Cite as

Study of spatial organization of chicken α-globin gene domain by 3c technique

  • A. A. GavrilovEmail author
  • S. V. Razin
Accelerated Publication


This work deals with 3C (Chromosome Conformation Capture) analysis of the chicken α-globin gene domain in embryonic erythrocytes and lymphoid cells. Ligation products were quantitatively analyzed by real-time PCR with TaqMan probes. It was found that in lymphoid cells, where α-globin gene is not active, the domain has a relatively extended configuration. In embryonic erythrocytes that transcribe αD and αA genes, simultaneous interaction of several domain elements was revealed including the major regulatory element, the erythroid-specific DNase I hypersensitive site at a distance of 9 kb upstream from the α-globin gene cluster (-9 DHS), promoter of the housekeeping gene CGTHBA, the αD-globin gene promoter, and the erythroid-specific enhancer located after the α-globin gene cluster. We suppose that such interaction is necessary to provide for the active transcription status of the chicken α-globin gene domains in erythroid cells.

Key words

chicken α-globin gene domain genome spatial organization 3C real-time PCR with TaqMan probes 



bacterial artificial chromosome


chromosome conformation capture


DNase I hypersensitive sites


major regulatory element


yeast artificial chromosome


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Copyright information

© MAIK Nauka 2008

Authors and Affiliations

  1. 1.Institute of Gene BiologyRussian Academy of SciencesMoscowRussia
  2. 2.Biological FacultyLomonosov Moscow State UniversityMoscowRussia

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