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Biochemistry (Moscow)

, Volume 73, Issue 11, pp 1176–1182 | Cite as

Changes in physicochemical parameters and α-crystallin expression in the lens during cataract development in OXYS rats

  • Yu. V. Rumyantseva
  • A. Zh. Fursova
  • L. A. Fedoseeva
  • N. G. KolosovaEmail author
Accelerated Publication

Abstract

The pathogenesis of cataract is associated with oxidative stress and with altered crystallin expression but it is still understood incompletely. In this study, the senescence-accelerated OXYS rats were used as a model. The first biomicro-scopic signs of cataract in OXYS rats were registered at the age of 1.5 months; at 3 months morbidity reached 90%, and at 6 months it reached 100%. Cataract manifestation progresses: at 24 months mature cataract was detected in 90% of eyes of OXYS rats, whereas in 80% of Wistar rat eyes only initial signs of this disease were detected. Analysis of lens redox-parameters has shown that in OXYS rats the intensity of tryptophan fluorescence is higher, the GSH content being higher at 2 months but during formation of mature cataract at 13, 18, and 24 months being lower than in Wistar rats. Decrease in solubility of OXYS rat lens proteins was observed at the age of 13 months. At the age of 3 months gene expression of αA-crystallin and αB-crystallin was 3-fold and 25% lower, respectively, than in Wistar rats. At the age of 14 months there was a 27-fold decrease in expression of αB-crystallin in OXYS rats and it became 21-fold lower than in control. Proteins are synthesized in lens epithelial cells and dystrophic changes in senile cataract result in decrease in structural protein expression. The changes observed in OXYS rats are evidently associated with the dystrophic changes in lens epithelium, which we have described earlier, and are consistent with the model of senile cataract.

Keys words

cataract oxidative stress α-crystallin expression senescence-accelerated OXYS rats 

Abbreviations

LPO

lipid peroxidation

MDA

malondialdehyde

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References

  1. 1.
    Truscott, R. J. (2006) in Prevention and Treatment of Age-Related Diseases (Rattan, S., and Kassem, M., eds.) Springer, N.Y., pp. 159–174.Google Scholar
  2. 2.
    Shinohara, T., White, H., Mulhern, M. L., and Maisel, H. (2007) Medical Hyp., 69, 669–677.CrossRefGoogle Scholar
  3. 3.
    Shang, F., Nowell, T., Jr., Gong, X., Smith, D. E., Dallal, G. E., Khu, P., and Taylor, A. (2002) Exp. Eye Res., 5, 109–111.CrossRefGoogle Scholar
  4. 4.
    Nishimoto, H., Uga, S., Miyata, M., Ishikawa, S., and Yamashita, K. (1993) Graefes Arch. Clin. Exp. Ophthalmol., 231, 722–728.PubMedCrossRefGoogle Scholar
  5. 5.
    Noriaki, N., Noriko, T., Akira, K., and Yoshimasan, I. (2006) Biol. Pharm. Bull., 29, 236–248.Google Scholar
  6. 6.
    Mori, M., Li, G., and Abe, I. (2006) J. Clin. Invest., 116, 395–404.PubMedCrossRefGoogle Scholar
  7. 7.
    Solov’eva, N. A., Morozkova, T. S., and Salganik, R. I. (1975) Genetika, 11, 63–71.PubMedGoogle Scholar
  8. 8.
    Kolosova, N. G., Lebedev, P. A., Aidagulova, S. V., and Morozkova, T. S. (2003) Byul. Eksp. Biol. Med., 136, 467–471.CrossRefGoogle Scholar
  9. 9.
    Kolosova, N. G., Lebedev, P. A., Fursova, A. Zh., Morozkova, T. S., and Gusarevich, O. G. (2003) Usp. Gerontol., 12, 143–148.Google Scholar
  10. 10.
    Marsili, S., Salganik, R. I., Albright, C. D., Freel, C. D., Johnsen, S., Peiffer, R. L., and Costello, M. J. (2004) Exp. Eye Res., 79, 595–612.PubMedCrossRefGoogle Scholar
  11. 11.
    Gusarevich, O. G., Kolosova, N. G., and Fursova, A. Zh. (2005) Rus. Med. Zh., 4, 186–189.Google Scholar
  12. 12.
    Kolosova, N. G., Trofimova, N. A., Shcheglova, T. V., and Sergeeva, S. V. (2005) Byul. Eksp. Biol. Med., 139, 397–999.CrossRefGoogle Scholar
  13. 13.
    Bobko, A. A., Sergeeva, S. V., Bagryanskaya, E. G., Markel, A. L., Khramtsov, V. V., Reznikov, V. A., and Kolosova, N. G. (2005) Biochem. Biophys. Res. Commun., 330, 367–370.PubMedCrossRefGoogle Scholar
  14. 14.
    Sergeeva, S. V., Bagryanskaya, E. G., Korbolina, E. E., and Kolosova, N. G. (2006) Exp. Gerontol., 41, 141–150.PubMedCrossRefGoogle Scholar
  15. 15.
    Kolosova, N. G., Shcheglova, T. V., Sergeeva, S. V., and Loskutova, L. V. (2006) Neurobiol. Aging, 27, 1289–1297.PubMedCrossRefGoogle Scholar
  16. 16.
    Tsentalovich, Y. P., Snytnikova, O. A., Forbes, M. D. E., Chernyak, E. I., and Morozov, S. V. (2006) Exp. Eye Res., 83, 1439–1445.PubMedCrossRefGoogle Scholar
  17. 17.
    Hawse, J. R., Hejtmancik, J. F., Huang, Q., Sheets, N. L., Hosack, D. A., Lempicki, R. A., Horwitz, J., and Kantorow, M. (2003) Molecular Vision, 9, 515–537.PubMedGoogle Scholar
  18. 18.
    Babizhayev, M. A., Deyev, A. I., Yermakova, V. N., Brikman, I. V., and Bours, J. (2004) Drugs R. D., 5, 125–139.PubMedCrossRefGoogle Scholar
  19. 19.
    Ellman, G. L. (1959) Arch. Biochem. Biophys., 82, 70–77.PubMedCrossRefGoogle Scholar
  20. 20.
    Esterbauer, H., and Cheeseman, K. H. (1990) Meth. Enzymol., 186, 407–420.PubMedCrossRefGoogle Scholar
  21. 21.
    Nolan, T., Hands, R. E., and Bustin, S. A. (2006) Nature Protocols, 1, 1559–1582.PubMedCrossRefGoogle Scholar
  22. 22.
    Vinson, J. A. (2006) Pathophysiology, 13, 151–162.PubMedCrossRefGoogle Scholar
  23. 23.
    Kolosova, N. G., Fursova, A. Zh., Lebedev, P. A., and Gusarevich, O. G. (2004) Oftalmol. Zh., 3, 237–242.Google Scholar
  24. 24.
    Aseychev, A. V., Sitarchuk, I. A., Deev, A. I., Aitmagambetov, M. T., and Vladimirov, Yu. A. (1994) in Study of Kinetics of Accumulation and Molecular Mass of Lens Fluorophores during Periods Preceding Cataract Development (Tikhonov, A. N., Sadovnichiy, V. A., et al., eds.) MSU Press, Moscow, pp. 22–27.Google Scholar
  25. 25.
    Deyev, A. I., Aseychev, A. V., Sitartchuk, I. A., Fedorenko, B. S., and Vladimirov, Yu. A. (1999) in Ocular Radiation Risk Assessment in Population Exposed to Environmental Radiation Contamination (Junk, A. K., et al., eds.) Kluwer Academic Publishers, Dorbrecht-Boston-London, pp. 81–88.Google Scholar
  26. 26.
    Ferrer, V., Gasca, E., Sastre, J., and Pallardo, F. V. (1990) Biochem. J., 269, 531–534.PubMedGoogle Scholar
  27. 27.
    Andley, U. P. (2007) Progr. Retinal Eye Res., 26, 78–98.CrossRefGoogle Scholar

Copyright information

© MAIK Nauka 2008

Authors and Affiliations

  • Yu. V. Rumyantseva
    • 1
  • A. Zh. Fursova
    • 1
  • L. A. Fedoseeva
    • 1
  • N. G. Kolosova
    • 1
    Email author
  1. 1.Institute of Cytology and GeneticsSiberian Branch of the Russian Academy of SciencesNovosibirskRussia

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