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Biochemistry (Moscow)

, Volume 73, Issue 10, pp 1114–1120 | Cite as

Quasi-equilibrium assumption in enzyme kinetics. Necessary and sufficient conditions and accuracy of its application for single-substrate reactions

  • P. V. VrzheshchEmail author
Article

Abstract

Steady-state kinetics of compulsory-ordered single-substrate irreversible and reversible enzyme reactions with two, three, and arbitrary number of intermediates were observed. Necessary and sufficient conditions for application of the quasi-equilibrium assumption and restrictions of this assumption were found in cases of two and three intermediates in the equilibrium segment. For all cases, accuracy of the quasi-equilibrium assumption was evaluated.

Key words

enzyme kinetics quasi-equilibrium assumption rapid equilibrium theory steady-state kinetics Cha method accuracy of assumption 

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Copyright information

© MAIK Nauka 2008

Authors and Affiliations

  1. 1.Faculty of Bioengineering and BioinformaticsLomonosov Moscow State UniversityMoscowRussia
  2. 2.International Biotechnological CenterLomonosov Moscow State UniversityMoscowRussia

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