Quasi-equilibrium assumption in enzyme kinetics. Necessary and sufficient conditions and accuracy of its application for single-substrate reactions
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Steady-state kinetics of compulsory-ordered single-substrate irreversible and reversible enzyme reactions with two, three, and arbitrary number of intermediates were observed. Necessary and sufficient conditions for application of the quasi-equilibrium assumption and restrictions of this assumption were found in cases of two and three intermediates in the equilibrium segment. For all cases, accuracy of the quasi-equilibrium assumption was evaluated.
Key wordsenzyme kinetics quasi-equilibrium assumption rapid equilibrium theory steady-state kinetics Cha method accuracy of assumption
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